节杆菌和腐熟鸡粪混施对Atrazine污染土壤特性影响  

Effects of Combined Application of Arthrobacter and Decomposed Chicken Manure on Soil Characteristics Contaminated by Atrazine

作　　者：李文帅 段玉春 范文艳[1] 韩文革[1] 吕清晗 吴皓菘 李明睿 李宝成 姜述君[1] Li Wenshuai;Duan Yuchun;Fan Wenyan;Han Wenge;Lv Qinghan;Wu Haosong;Li Minrui;Li Baocheng;Jiang Shujun(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学农学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2024年第3期18-24,共7页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省农垦总局科技攻关(HNK135-02-05)。

摘　　要：为明确节杆菌菌株MSD6与腐熟鸡粪混用修复阿特拉津污染土壤的潜力。采用模拟阿特拉津污染土壤,设5个处理:CK1,空白对照,无添加林地土;CK2,药剂对照,仅添加阿特拉津;T1,阿特拉津+菌株MSD6(5×10^(8)CFU·kg^(-1));T2,阿特拉津+腐熟鸡粪50 g;T3,阿特拉津+菌株MSD6(5×10^(8)CFU·kg^(-1))+腐熟鸡粪50 g。结果表明,菌株MSD6和腐熟鸡粪混施能显著降低土壤阿特拉津含量,并能较好恢复阿特拉津引起的土壤微生物量碳和土壤酶活性的降低。培养40 d后,T3处理的阿特拉津降解率为92.8%,土壤微生物量碳较CK2升高206.8%。T3处理的土壤过氧化氢酶、碱性磷酸酶、蔗糖酶和脲酶活性显著高于CK2处理。综上所述,节杆菌菌株MSD6与腐熟鸡粪混用能有效地改善阿特拉津污染土壤的生物学特性。To clarify the potential of using Arthrobacter sp strain MSD6 mixed with decomposed chicken manure for remediation of atrazine contaminated soil,Atrazine contaminated soil was simulated in laboratory.Five treatments with four repetitions each were applied on the experiment including CK1(control,no additional woodland soil),CK2(only added atrazine),T1(atrazine with 5×10^(8) CFU·kg^(-1) of added strain MSD6),T2(atrazine with 50 g of added decomposed chicken manure),T3(atrazine with 5×10^(8) CFU·kg^(-1) MSD6 of added strain and 50 g of decomposed chicken manure).The results indicated that the mixed application of strain MSD6 and decomposed chicken manure could significantly reduce soil atrazine content and had a good recovery effect on the reduction of soil microbial biomass carbon and soil enzyme activity caused by atrazine.After 40 d culture,the degradation rate of atrazine in T3 treatment was 92.8%and the soil microbial carbon content of T3 treatment was 206.8%higher than that of CK2.T3 treatment could better alleviate the inhibition of atrazine on enzyme activities of catalase,alkaline phosphatase,sucrase and urease.In conclusion,strain MSD6 mixed with decomposed chicken manure could effectively restore the biological characteristics of atrazine contaminated soil.

关 键 词：Arthrobacter sp 腐熟鸡粪 阿特拉津 土壤微生物碳 土壤酶 

分 类 号：Q-9[生物学]