响应面优化法制备“腾药”乳膏及其镇痛作用研究  

Optimization of the Preparation of“Teng Medicine”(腾药)Cream by Response Surface Optimization Methodology and a Preliminary Study on Its Analgesic Effect

作　　者：潘旭[1] 王洁煊 曲舒显 潘琦[3] 张蕊[1] 李志远 霍梦逸 PAN Xu;WANG Jiexuan;QU Shuxian;PAN Qi;ZHANG Rui;LI Zhiyuan;HUO Mengyi(Beijing Jishuitan Hospital Affiliated to Capital Medical University,Beijing 100035,China;Beijing University of Traditional Chinese Medicine,Beijing 102401,China;Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100005,China)

机构地区：[1]首都医科大学附属北京积水潭医院,北京100035 [2]北京中医药大学,北京102401 [3]北京医院/国家老年医学中心/中国医学科学院老年医学研究院,北京100005

出　　处：《中医药导报》2024年第5期65-70,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点研发计划(2020YFC2009004)。

摘　　要：目的:将“腾药”熥剂制备为“腾药”乳膏,并对比研究“腾药”熥剂和“腾药”乳膏的镇痛作用,为“腾药”熥剂的开发利用提供参考。方法:通过单因素筛选和Box-Behnken响应面法,筛选出“腾药”乳膏基质的最佳处方工艺,以乳膏的外观均匀性、离心稳定性、涂布延展性、耐寒稳定性、耐热稳定性5个方面作为评价指标对制备的“腾药”乳膏进行综合评价;运用醋酸扭体法、甲醛致痛模型、热板法对比研究“腾药”熥剂和“腾药”乳膏的镇痛作用。结果:最佳“腾药”乳膏处方为载药量0.29 g,硬脂酸用量0.91 g,单硬脂酸甘油酯用量0.50 g。“腾药”乳膏与“腾药”熥剂在醋酸扭体法中疼痛抑制率分别为28.77%、28.74%;甲醛致痛模型实验中“腾药”乳膏第I相、第Ⅱ相小鼠平均累计舔足时间分别为28.83 s、36.00 s;“腾药”熥剂第I相、第Ⅱ相小鼠平均累计舔足时间分别为30.17 s、38.25 s;热板法实验中“腾药”乳膏痛阈提高百分率为90.54%,“腾药”熥剂痛阈提高百分率为69.87%。结论:“腾药”乳膏制备工艺可行,其镇痛效果与“腾药”熥剂相当。Objective:"Teng medicine"hot pack agent was produced as"Teng medicine"cream,and the analgesic effect of"Teng medicine"hot pack agent and"Teng medicine"cream were compared.It is a reference for the development and use of"Teng medicine".Methods:Optimum formulation of the"Teng medicine"cream base was determined by single-factor screening and Box-Behnken response surface methodology.The five aspects of the cream,including uniformity of appearance,centrifugal stability,spreadability,cold stability and heat stability,were used as evaluation indexes to comprehensively evaluate the prepared"Teng medicine"creams.The analgesic effects of"Teng medicine"hot pack agent and"Teng medicine"cream were compared and studied using the acetic acid torsion method,formaldehyde pain model and hot plate method.Results:The optimal prescription for"Teng medicine"cream is 0.29 g of drug,0.91 g of stearic acid and 0.50 g of glycerylmonostearate.The pain inhibition rate of"Teng medicine"cream and"Teng medicine"hot pack agent was 28.77%and 28.74%respectively in the acetic acid torsion method.In the formaldehyde pain model,the average cumulative foot licking time for mice in phaseⅠand phaseⅡof"Teng medicine"cream was 28.83 s and 36.00 s respectively;The average cumulative foot licking time for mice in phaseⅠand phaseⅡof"Teng medicine"hot pack agent was 30.17 s and 38.25 s respectively.In the hot plate experiment,the final percentage increase in pain threshold was 90.54%for"Teng medicine"cream and 69.87%for"Teng medicine"hot pack agent.Conclusion:The process of preparing"Teng medicine"cream is feasible and its analgesic effect is comparable to that of"Teng medicine"hot pack agent.

关 键 词：腾药 乳膏 熥剂 响应面法 镇痛作用 

分 类 号：R283.6[医药卫生—中药学]