短串联重复序列扫描技术对无特定病原体新西兰兔遗传结构的分析  

Analyzsis of Population Genetic Structure of Specific Pathogen Free New Zealand Rabbit by Short Tandem Repeat Scanning Technology

作　　者：刘科[1] 黄小红[1] 陈傍柱 刘天平 张富发[1] 陈华财[1] 顾为望[3] 王刚[1] LIU Ke;HUANG Xiaohong;CHEN Bangzhu;LIU Tianping;ZHANG Fufa;CHEN Huacai;GU Weiwang;WANG Gang(Guangdong Medical Laboratory Animal Center,Foshan Guangdong 528248;School of Biotechnology and Health Sciences,Wuyi University,Jiangmen Guangdong 529020;Institute of Comparative Medicine&Laboratory Animal Center,Southern Medical University,Guangzhou Guangdong 510515)

机构地区：[1]广东省医学实验动物中心,广东佛山528248 [2]五邑大学/生物科技与大健康学院,广东江门529020 [3]南方医科大学/比较医学研究所暨实验动物中心,广东广州510515

出　　处：《广东畜牧兽医科技》2024年第3期33-38,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省科技计划项目(2019A030317012,2021B1212040016)。

摘　　要：应用STR扫描技术,对广东省医学实验动物中心SPF级新西兰兔进行遗传检测和分析,以掌握该兔群体遗传结构,为制定繁殖育种方案提供依据,并为兔遗传分析方法提供参考。试验采集33只种兔耳静脉血,提取基因组DNA,选用多态丰富的23个微卫星位点对基因组DNA进行PCR扩增,扩增产物进行STR扫描,扫描结果运用popgene32软件分析群体观察等位基因数、有效等位基因数、香隆指数和有效杂合度等遗传结构信息。结果显示,全部23个微卫星位点PCR扩增良好,兔群体平均观测等位基因数为1.5217,平均有效等位基因数为1.2786,平均香隆指数为0.2442,平均有效杂合度为0.1564。初步掌握了该SPF级新西兰兔群体遗传结构。结果表明,STR扫描技术及选用的23个微卫星位点适用于兔群体遗传结构检测和分析,将为兔遗传分析方法提供参考和依据。The STR scanning technology was used to conduct genetic detection and analysis on SPF New Zealand rabbits in Guangdong Medical Experimental Animal Center,so as to master the genetic structure of the rabbit population,provide basis for formulating breeding plans,and provide reference for rabbit genetic analysis methods.The venous blood from the ears of 33 rabbits was collected to extract genomic DNA.23 microsatellite loci with rich polymorphism were selected for PCR amplification of genomic DNA.The amplified products were done by STR scanning.The scanning results were done genetic structure analysis of the population include observe the number of alleles,effective alleles,vanillin index and effective heterozygosity by popgene32 software.The results showed that the PCR amplification of all 23 microsatellite loci were well,the average observed allele of the rabbit population is 1.5217,the average effective allele count is 1.2786,the average Shannon index is 0.2442,and the average effective heterozygosity is 0.1564.The genetic structure of the SPF New Zealand rabbit population was preliminarily mastered.The results indicated that the STR scanning technique and the 23 microsatellite loci selected were suitable for the detection and analysis of the genetic structure of rabbit population,and would provide reference and basis for the rabbit genetic analysis methods.

关 键 词：新西兰兔 SPF STR扫描 遗传分析 

分 类 号：S813.1[农业科学—畜牧学]