三种消毒剂对鹅星状病毒杀灭效果的评价  

Evaluation on the Effect of Three Disinfectants on Goose Astrovirus

作　　者：邝瑞欢 黄允真 李林林 向勇 翟颀 孙敏华 李珂 信爱国 李海贤 董嘉文 KUANG Ruihuan;HUANG Yunzhen;LI Linlin;XIANG Yong;ZHAI Qi;SUN Minhua;LI Ke;XIN Aiguo;LI Haixian;DONG Jiawen(Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases,Ministry of Agriculture and Rural Affairs Key Laboratory of Livestock Disease Prevention and Treatment of Guangdong Province/Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640;Department of Poultry Husbandry and Disease Research,Yunnan Animal Science and Veterinary Institute,Kunming Yunnan 650224;Guangzhou Wufeng Animal Health Products Co.,Ltd,Guangzhou Guangdong 510640)

机构地区：[1]广东省农业科学院动物卫生研究所/农业农村部禽流感等家禽重大疾病防控重点实验室/广东省畜禽疫病防治研究重点实验室,广东广州510640 [2]云南省畜牧兽医科学院养禽与禽病研究所,云南昆明650224 [3]广州五丰动物保健品有限公司,广东广州510640

出　　处：《广东畜牧兽医科技》2024年第3期88-92,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：“十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目(2022SDZG02);科技创新战略专项资金(高水平农科院建设)(R2020PY-JX014;R2020QD-049);广东省现代农业产业技术体系创新团队(2023KJ137);云南省廖明专家工作站(202105AF150077);江门市基础与应用基础项目(2021030102450004518)。

摘　　要：为了解不同成分的消毒剂对鹅星状病毒的杀灭效果,该试验选择复方戊二醛、二氯异氰脲酸钠粉、聚维酮碘溶液进行杀灭效果评估,以指导临床实践中的消毒灭源工作。经对三种消毒剂在10℃、25℃、37℃下不同稀释比例、不同作用时间对鹅星状病毒的杀灭效果进行实验室评价,结果显示:在室温环境下(25℃),作用时间为1 min、5 min和10 min,复方戊二醛1∶80倍稀释可完全杀灭鹅星状病毒(104.0 EID50),而复方戊二醛1∶300倍稀释及其他两种消毒剂在推荐使用浓度下均未能有效杀灭鹅星状病毒。对于尿囊液里高含量(104.0 EID50)的鹅星状病毒,1∶80倍稀释的复方戊二醛溶液在10℃时需要作用10 min才能有效杀灭星状病毒,但尿囊液里低含量(103.0 EID50)的鹅星状病毒在此浓度下作用1 min就能被完全杀灭。当温度达到25℃时,1∶80倍稀释的复方戊二醛溶液1 min即可杀灭鹅星状病毒;1:160倍稀释的复方戊二醛溶液则需要10 min才能完全杀灭鹅星状病毒。继续升高温度至37℃,1∶80和1∶160倍稀释的复方戊二醛溶液与鹅星状病毒作用1 min即可起到有效杀灭效果。综上所述,采用1∶80倍稀释的复方戊二醛杀灭鹅星状病毒(104.0 EID50),在10℃、25℃和37℃作用的最短时间分别是10 min、1 min和1 min。该研究明确了三种不同消毒剂首次用于消毒,在不同温度和作用时间下对鹅星状病毒的杀灭效果,为鹅星状病毒的生物安全防控提供参考。In order to understand the killing effect of disinfectants of different components on Goose Astrovirus,compound glutaraldehyde,sodium dichloroisocyanurate powder and povidone-iodine solution were selected for the evaluation of the killing effect to guide the disinfection and source elimination in clinical practice.After laboratory evaluation of the killing effect of the three disinfectants on Goose Astrovirus at different dilution ratios and different reaction times at 10℃,25℃ and 37℃,the results showed that at room tempreture(25℃),only 1∶80-fold dilution of compound glutaraldehyde among the three disinfectants could completely kill Goose Astrovirus at 1 min,5 min and 10 min of reaction time,while compound glutaraldehyde 1∶300-fold dilution and other two disinfectants of recommended concentration failed to effectively kill Goose Astrovirus.For high content of Goose Astrovirus(104.0EID50),1∶80-fold diluted compound glutaraldehyde solution needs to work for 10 min at 10℃ to effectively kill Goose Astrovirus,but low content of Goose Astrovirus(103.0EID50)can be completely killed at this concentration.When the temperature reaches 25℃,1∶80 times diluted compound glutaraldehyde solution can kill Goose Astrovirus for 1 min;However,a 1∶160-fold dilution of compound glutaraldehyde solution takes 10 min to completely kill Goose Astrovirus.Continue to increase the temperature to 37℃,1∶80 and 1∶160 times diluted compound glutaraldehyde solution and Goose Astrovirus for 1 min can play an effective killing effect.In summary,the shortest time for killing Goose Astrovirus(104.0EID50)with 1∶80 compound glutaraldehyde at 10℃,25℃ and 37℃ was 10 min,1 min and 1 min,respectively;This study clarifies the killing effect of different disinfectants on Goose Astrovirus at different temperatures and reaction times for the first time,and provides a reference for the biosecurity prevention and control of Goose Astrovirus.

关 键 词：鹅星状病毒 消毒 复方戊二醛 异氰脲酸钠 聚维酮碘 

分 类 号：S852.657[农业科学—基础兽医学]