基于“辨色论质”的新疆紫草质量评价研究  

Study on quality control of Arnebiae Radix based on“color discrimination grading”

作　　者：连超杰[1] 戴胜云 刘杰[1] 过立农[1] 乔菲[1] 杨锐[1] 王会娟 郑健[1] 刘杰 LIAN Chao-jie;DAI Sheng-yun;LIU Jie;GUO Li-nong;QIAO Fei;YANG Rui;WANG Hui-juan;ZHENG Jian;LIU Jie(National Institutes for Food and Drug Control,Beijing 102629,China;Yili Institute of Inspection,Testing and Certification,Yili 835000,China)

机构地区：[1]中国食品药品检定研究院,北京102629 [2]伊犁哈萨克自治州检验检测认证研究院,伊型835000

出　　处：《药物分析杂志》2024年第5期766-771,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：中国食品药品检定研究院学科带头人培养基金(2021X4);国家药品监督管理局药品监管科学体系建设重点项目“新技术新方法在中药质量控制中的应用”(RS2024Z006-116);新疆维吾尔自治区药品监督管理局智力援疆创新拓展人才计划--“新疆特色民族药研究(以新疆紫草为例)团队”项目。

摘　　要：目的:测量紫草的色泽,测定紫草中6个主要紫色素类成分(乙酰紫草素、β-乙酰氧基异戊酰阿卡宁、去氧紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁、异戊酰紫草素)的含量,研究新疆紫草色泽与6个主要紫色素成分含量的相关性。方法:采用分光测色仪测定样品粉末的L、a、b值用于表征紫草的色泽。国际照明委员会(CIE)制定了Lab颜色模型,是人类视觉的数字化描述,L值越大表示亮度越大,a值增大表示偏红减小表示偏绿,b值增大表示偏黄减小表示偏蓝;采用高效液相色谱法(HPLC)测定紫色素类成分的含量,使用SPSS软件计算L、a、b值与6个主要紫色素含量的相关程度。结果:135批样品乙酰紫草素的含量为0.01%~3.39%,β-乙酰氧基异戊酰阿卡宁含量为0.00%~1.95%,去氧紫草素含量为0.00%~0.23%,异丁酰紫草素含量为0.01%~1.13%,异戊酰紫草素含量为0.02%~2.88%,β,β’-二甲基丙烯酰阿卡宁含量为0.01%~2.17%。紫草药材的L(黑_白)色度值与乙酰紫草素、β-乙酰氧基异戊酰阿卡宁和异丁酰紫草素3个成分的含量呈显著负相关关系,斯皮尔曼相关系数在-0.138和-0.222之间;a(绿_红)色度值与β-乙酰氧基异戊酰阿卡宁、去氧紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁、异戊酰阿卡宁的含量均呈现显著的正相关,斯皮尔曼相关系数在0.176和0.355之间;b(蓝-黄)色度值与乙酰紫草素、去氧紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁、异戊酰阿卡宁均呈现显著的相关性,其中,与β,β’-二甲基丙烯酰阿卡宁呈正相关,系数为0.290,与其余4个成分呈负相关,系数在-0.325和-0.633之间。结论:建议紫草(新疆紫草)的含量测定项修订为β,β’-二甲基丙烯酰阿卡宁不得少于0.30%并且异丁酰紫草素不得少于0.29%。Objective:To determine the color of Arnebiae Radix,and the contents of six main purple pigment components(acetylshikonin,β-acetoxyisovalerylalkannin,deoxyshikonin,isobutylshikonin,β,β'-dimethylacrylalkannin and isovalerylshikonin)in Arnebiae Radix,and to study on the correlation between the color of Arnebiae Radix and the contents of six main purple pigment components.Methods:The L,a,and b values of the sample powder were determined using a spectrophotometer to characterize the color of Arnebiae Radix.The International Commission on Illumination(CIE)had developed a Lab color model,which was a digital description of human vision.A higher L value indicated greater brightness,a higher a value indicated redness and a lower a value indicates greenness,and a higher b value indicated yellowing and a lower b value indicates blueness.The contents of purple pigment components were determined using high performance liquid chromatography(HPLC),and the correlation between L,a,and b values and the contents of six main purple pigments was calculated using SPSS software.Results:The contents of acetylshikonin in 135 batches of samples ranged from 0.01%to 3.39%.The contents ofβ-acetoxyisovalerylalkannin ranged from 0.00%to 1.95%.The contents of deoxyshikonin ranged from 0.00%to 0.23%.The contents of isobutylshikonin ranged from 0.01%to 1.13%.And the contents of isovalerylshikonin ranged from 0.02%to 2.88%.The contents ofβ,β'-dimethylacrylalkannin ranged from 0.01%to 2.17%.There was a significant negative correlation between the contents of acetylshikonin,β-acetoxyisovalerylalkannin and isobutylshikonin and the L(black_white)chromaticity value of Arnebiae Radix,with a Spearman correlation coefficients between-0.138 and-0.222.The chromaticity value of a(red_green)was related to the five components other than acetylshikonin,which wereβ-acetoxyisovalerylalkannin,deoxyshikonin,isobutylshikonin,β,β'-dimethylacrylalkannin,and isovalerylshikonin,with a spearman correlation coefficients between 0.176 and 0.355;b(blue_yellow)chrom

关 键 词：紫草 色泽 乙酰紫草素 β-乙酰氧基异戊酰阿卡宁 去氧紫草素 异丁酰紫草素 β β’-二甲基丙烯酰阿卡宁 异戊酰紫草素 相关性分析 

分 类 号：R917[医药卫生—药物分析学]