新疆紫草野生品与栽培品的质量比较研究  

Comparative study on the quality of wild and cultivated Arnebiae Radix

作　　者：黄瑞 戴胜云 吴东雪 马晓军 刘杰[1] 过立农[1] 道尔加拉 敬松 马双成[1] 郑健[1] HUANG Rui;DAI Sheng-yun;WU Dong-xue;MA Xiao-jun;LIU Jie;GUO Li-nong;Dao-er-jia-la;JING Song;MA Shuang-cheng;ZHENG Jian(National Institutes for Food and Drug Control,Beijing 102629,China;China Pharmaceutical University,Nanjing 211198,China;Yili Institute of Inspection,Testing and Certification,Yili 835000,China;Xinjiang Delikai Agriculture and Animal Husbandry Science and Technology Development Co.,Ltd.,Urumqi 830000,China;Xinjiang Xinjia Agricultural Science and Technology Development Co.,Ltd.,Ili 835400,China)

机构地区：[1]中国食品药品检定研究院,北京102629 [2]中国药科大学,南京211198 [3]伊犁哈萨克自治州检验检测认证研究院,伊犁835000 [4]新疆德立凯农牧科技发展有限公司,乌鲁木齐830000 [5]新疆欣嘉农业科技开发有限公司,伊犁835400

出　　处：《药物分析杂志》2024年第5期783-795,共13页Chinese Journal of Pharmaceutical Analysis

基　　金：中国食品药品检定研究院学科带头人培养基金(2021X4);新疆维吾尔自治区药品监督管理局智力援疆创新拓展人才计划--“新疆特色民族药研究(以新疆紫草为例)团队”项目。

摘　　要：目的:对新疆紫草野生品与栽培品的质量进行比较研究,包括性状对比和结合化学计量学分析新疆紫草野生品与3个不同地区栽培品的差异性成分。方法:分别采集新疆紫草野生品与栽培品,对其性状进行对比;采用Waters ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱,以乙腈-0.05%甲酸水为流动相,检测波长275 nm,流速0.2 mL·min^(-1),对48批野生品和栽培品中的右旋紫草素、乙酰紫草素、β-乙酰氧基异戊酰阿卡宁、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁和异戊酰紫草素6个成分进行含量测定,并结合主成分分析(PCA)和正交偏最小二乘-判别分析(OPLS-DA)分析野生品和栽培品中的差异性成分。结果:新疆紫草野生品与栽培品在性状上存在较大差异,建立的含量测定方法线性关系良好,r>0.999,平均加样回收率93.4%~102.9%,RSD<3.0%,不同批次新疆紫草中6个成分含量差异较大,其中野生品中右旋紫草素、β-乙酰氧基异戊酰阿卡宁含量均明显高于栽培品,说明野生品与栽培品还存在一定的差异;建立的PCA模型可区分野生品和栽培品,且通过OPLS-DA确定了异丁酰紫草素和β,β’-二甲基丙烯酰阿卡宁是野生品和栽培品中的2个差异性成分。结论:通过对比野生品和栽培品的木心大小、栓皮卷曲程度以及特异性气味,可对二者进行初步鉴别;建立的含量测定方法重复性好,专属性强,稳定可行;确定了新疆紫草野生品与3个不同地区栽培品的差异性成分,为新疆紫草的质量控制提供依据,为扩大紫草药源提供了思路。Objective:To compare the quality of wild and cultivated Arnebiae Radix,using macroscopic investigation and chemometric analysis of the different components in wild and cultivated Arnebiae Radix from three different habitats.Methods:Wild and cultivated Arnebiae Radix were collected and their macroscopic features were compared.Using the ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)column,with acetonitrile-0.05%formic acid water as the mobile phase,the contents of D-shikonin,acetylshikonin,β-acetoxyisovalerylshikonin,isobutyrylshikonin,β,β'-dimethylacrylalkannin and isovalerylshikonin in 48 batches of wild and cultivated Arnebiae Radix were determined.The detection wavelength was 275 nm and the flow rate was 0.2 mL·min^(-1).PCA and OPLS-DA were performed to reveal the differential components of wild and cultivated Arnebiae Radix.Results:There were great differences in macroscopic features of wild and cultivated Arnebiae Radix,and the linear relationship between the contents of six naphthoquinone components was good.The correlation coefficients were above 0.999,the average recovery rates were 93.4%-102.9%,and the RSDs were less than 3.0%.The contents of six components in different batches of wild and cultivated Arnebiae Radix were quite different,and the contents of D-shikonin and acetylshikonin in wild products were significantly higher than those in cultivated products,indicating that there were still certain differences between wild products and cultivated products.The PCA model established could distinguish wild products and cultivars,and two differentiating components in wild products and cultivars were revealed by OPLS-DA,namely isobutyryl shikonin,β,β'-dimethylacrylalkannin.Conclusion:By comparing the core size,cork curl degree and specific odor of wild and cultivated products,the two can be identified.The established content determination method is repeatable,specific,stable and feasible.The differential components in wild and cultivated Arnebiae Radix in three different regions are identified,which pr

关 键 词：新疆紫草 栽培品 野生品 差异性分析 β β’-二甲基丙烯酰阿卡宁 β-乙酰氧基异戊酰阿卡宁 异丁酰紫草素 

分 类 号：R917[医药卫生—药物分析学]