一测多评法测定艾叶中7个黄酮类成分的含量  

Simultaneous determination of seven flavanoids in Artemisiae Argyi Folium by quantitative analysis of multi-components by single marker

作　　者：孙立秋 王丹[1] 赵英楠 时志春 李军[1] 王金兰[1] 赵明[1] 张树军[1] SUN Li-qiu;WANG Dan;ZHAO Ying-nan;SHI Zhi-chun;LI Jun;WANG Jin-lan;ZHAO Ming;ZHANG Shu-jun(College of Chemistry and Chemical Engineering,Qiqihar University,Heilongjiang Industrial Hemp Processing Technology Innovation Center,Technology Innovation Center of Industrial Hemp for State Market Regulation,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院黑龙江省工业大麻加工技术创新中心国家市场监管技术创新中心(工业大麻),齐齐哈尔161006

出　　处：《药物分析杂志》2024年第5期806-815,共10页Chinese Journal of Pharmaceutical Analysis

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(145109512)。

摘　　要：目的:建立同时测定艾叶中7个黄酮类成分(5-羟基-6,7,3’,4’-四甲氧基黄酮、芹菜素、高车前素、山柰酚、棕矢车菊素、异泽兰黄素、蔓荆子黄素)的一测多评含量测定方法(QAMS法)。方法:采用高效液相色谱法,Agilent ZORBAX SB-C18色谱柱(150 mm×4.6 mm,5μm),以乙腈-0.2%磷酸水溶液为洗脱流动相,进行梯度洗脱,流速1.0 mL·min^(-1),检测波长350 nm,柱温30℃。以异泽兰黄素为内参物,建立与其他6个黄酮类成分的相对校正因子,并计算7个待测成分的含量,实现一测多评。同时与外标法进行比较,以验证QAMS法的准确性和可行性。结果:在一定的线性范围内,异泽兰黄素与5-羟基-6,7,3’,4’-四甲氧基黄酮、芹菜素、高车前素、山柰酚、棕矢车菊素、蔓荆子黄素的相对校正因子值分别为0.958、1.387、1.000、0.950、0.957、1.297(相对校正因子的RSD<2.0%),并与常规外标一点法比较,20批艾叶中5-羟基-6,7,3’,4’-四甲氧基黄酮、芹菜素、高车前素、山柰酚、棕矢车菊素、异泽兰黄素、蔓荆子黄素的含量测定结果分别为0.0314~0.6235、0.0009~0.0926、0.0206~0.1707、0.0110~0.1847、0.0117~0.8640、0.2532~2.5550、0.0156~0.2507 mg·g^(-1)。结论:以异泽兰黄素为内参物,建立艾叶中7个黄酮类成分的QAMS法准确、可行,可用于艾叶的定量分析及质量控制。Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)for simultaneous determination of 7 flavanoids(5-hydroxy-6,7,3',4'-tetramethoxyflavone,apigenin,hispidulin,kaempferol,jaceosidin,eupatilin and casticin)in Artemisiae Argyi Folium.Methods:The HPLC system consisted of the Agilent ZORBAX SB-C_(18)column(150 mm×4.6 mm,5μm)column with gradient elution of acetonitrile and 0.2%phosphoric acid as the mobile phase at a flow rate of 1.0 mL·min^(-1),a detection wavelength of 350 nm,and a column temperature of 30℃.Eupatilin was selected as the internal reference substance,the relative correction factors between eupatilin and the other 6 flavanoids were established,and the contents of these 7 constituents in samples were calculated to realize QAMS.At the same time,compared with the external standard method to verify the accuracy and feasibility of the QAMS method.Results:Within a certain linear range,the relative correction factors between eupatilin and 5-hydroxy-6,7,3',4'-tetramethoxyflavone,apigenin,hispidulin,kaempferol,jaceosidin as well as casticin were 0.958,1.387,1.000,0.950,0.957 and 1.297,respectively(RSDs of RCFs were less than 2.0%).The contents of 5-hydroxy-6,7,3',4'-tetramethoxyflavone,apigenin,hispidulin,kaempferol,jaceosidin,eupatilin,casticin in 20 batches of Artemisiae Argyi Folium were 0.0314-0.6235 mg·g^(-1),0.0009-0.0926 mg·g^(-1),0.0206-0.1707 mg·g^(-1),0.0110-0.1847 mg·g^(-1),0.0117-0.8640 mg·g^(-1),0.2532-2.5550 mg·g^(-1)and 0.0156-0.2507 mg·g^(-1),respectively.Conclusion:Using eupatilin as the internal reference,QAMS method for 7 flavanoids is established.The method is accurate and reliable,and can be used for quality control and quantitative analysis of Artemisiae Argyi Folium.

关 键 词：一测多评 相对校正因子 高效液相色谱 艾叶 黄酮 异泽兰黄素 

分 类 号：R917[医药卫生—药物分析学]