基于特征图谱、化学计量学和多指标成分测定的不同商品规格葛花质量差异分析  

Quality differences of Puerariae Flos with different commercial specifications based on HPLC characteristic chromatogram,chemometrics and multi-index component determination

作　　者：张立军 李鹏 李隆海 张新玥 马冬妮 张转平 胡芳弟[1] 王磊 柯红梅 李鹏 刘丽 吴春燕 李小安 ZHANG Li-jun;LI Peng;LI Long-hai;ZHANG Xin-yue;MA Dong-ni;ZHANG Zhuan-ping;HU Fang-di;WANG Lei;KE Hong-mei;LI Peng;LIU Li;WU Chun-yan;LI Xiao-an(School of Pharmacy,Lanzhou University,Lanzhou 730000,China;Ankang Inspection and Detection Center of Food and Drug Control,Ankang 725000,China;Shangluo Drug Inspection Center,Shangluo 726000,China;Xunyang City Jinzhai Town Agricultural Comprehensive Service Station,Xunyang 725751,China;Beijing University of Chinese Medicine,Beijing 100029,China;Dunhuang Hospital of Gansu Province,Dunhuang 736200,China;Ankang Tianyuan Plant Extraction Co.,Ltd.,Ankang 725000,China)

机构地区：[1]兰州大学药学院,兰州730000 [2]陕西省安康市食品药品检验检测中心,安康725000 [3]商洛市药品检验所,商洛726000 [4]旬阳市金寨镇农业综合服务站,旬阳725751 [5]北京中医药大学中药学院,北京1000294 [6]甘肃省敦煌市医院,敦煌736200 [7]安康市天源植物提取有限公司,安康725000

出　　处：《药物分析杂志》2024年第5期903-911,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：陕西省社会发展科技攻关项目(2015SF286);安康市社会发展科技攻关项目(AK2021-SF-01);陕西省药品科学监管和监管科学研究项目(SXYJ202317)。

摘　　要：目的:建立能有效区分葛花(Pueraria lobata(Willd.)Ohwi)、粉葛花(Pueraria thomsonii Benth.)及其混淆品种的特征图谱和多指标成分测定分析方法,用于葛花药材的整体质量控制。方法:采用高效液相色谱法(HPLC)对不同商品规格葛花中主要化学成分进行分析,确定最佳色谱条件,建立特征图谱;利用主成分分析(PCA)和偏最小二乘判别分析(PLS-DA)对不同商品规格葛花特征图谱数据进行分析,筛选确认差异性标志物;通过与对照品比对,对差异性标志物进行指认,进行定量分析,并分析测定结果。结果:建立了葛花、粉葛花、葛麻姆花及紫藤花的特征图谱,分别匹配了18、27、18及8个共有峰,指认出葛根素(Pu)、大豆苷(Da)、大豆苷元(Dae)、染料木苷(Ge)、染料木素(Gee)、葛花苷(Ka)、glycitin(Gl)、tectoridin(Td)、tectorigenin(Tg)及tectorigenin-7-O-xylosylglucoside(Tx)10个色谱峰,并同时建立了上述10个成分的含量测定方法。PCA和PLS-DA结果可将4种不同商品规格葛花样品明显区分,以Gl、Tx、Ge、Td、Ka及未知化合物(6号峰)为差异性标志物。葛花、粉葛花所含成分明显区别其混淆品,葛花中Ka含量为粉葛花的10倍,Ge含量为粉葛花的50%,未检测到Da、Gl。结论:所建立的特征图谱结合化学计量学及多指标成分测定分析方法简便、可行,可客观、有效地用于葛花药材质量控制,作为修改葛花质量标准提供参考。Objective:To establish HPLC characteristic chromatogram and multi-index component determination methods,so as to effectively distinguish Pueraria lobata(Willd.)Ohwi(GH),Pueraria thomsonii Benth(FGH)and their confusing species,and to evaluate the quality of Puerariae Flos.Methods:HPLC was used to analyze the chemical components from different Puerariae Flos with different commercial specifications.The chromatographic conditions were optimized,and the characteristic chromatogram was established.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)analysis were used to analyze the characteristic chromatogram data of Puerariae Flos with different commercial specifications and to select the differential markers of Puerariae Flos.The differential markers were identified by comparing them with the reference substance,and their contents were determined and the determination results were analyzed.Results:HPLC characteristic chromatograms of GH,FGH and their confusing species were established,and a total of 18,27,18 and 8 common peaks were matched in GH,FGH,Pueraria lobata var.montana(GMM)and Wisteria sinensis(Sims)Sweet(ZTH)respectively.Ten chromatographic peaks were identified,including puerarin(Pu),daidzin(Da),daidzein(Dae),genistin(Ge),genistein(Gee),kakkalide(Ka),glycitin(Gl),tectoridin(Td),tectorigenin(Tg)and tectorigenin-7-O-xylosylglucoside(Tx),and HPLC method for the determination of the above ten components was established simultaneously.The results of PCA and PLS-DA could distinguish four kinds of samples with different commercial specifications.Gl,Tx,Ge,Td,Ka and one unknown compound 6 were the components with significant differences between the samples with four different commodity specifications.The chemical components of GH and FGH were obviously different from their adulterants.The content of Ka in GH were 10 times as much as that in FGH,and the content of Ge was half as much as that in FGH.Da,Gl was not detected in GH.Conclusion:The established characteristic chromatogram

关 键 词：葛花 粉葛花 混淆品 特征图谱 化学计量学 质量控制 差异性标志物 葛花苷 染料木苷 

分 类 号：R917[医药卫生—药物分析学]