益智仁对力竭运动后小鼠心肌损伤的保护机制研究  

Protective effect and mechanism of Alpiniae oxyphylla fructus on myocardial injury after exhaustive exercise in mice

作　　者：温涛 徐百超[2,3] 王加猛 吴昊霖 骆丁 肖曼 WEN Tao;XU Baichao;WANG Jiameng;WU Haolin;LUO Ding;XIAO Man(International School of Public Health and One Health,Hainan Medical University,Haikou,Hainan 571199,China;不详)

机构地区：[1]海南医学院公共卫生与全健康国际学院,海南海口571199 [2]海南医学院海南省运动与健康促进重点实验室 [3]海南医学院体育部 [4]延安大学体育学院,陕西延安716000 [5]海南医学院衰老与肿瘤国际研究中心 [6]急救与创伤研究教育部重点实验室,海南医学院第一附属医院,海南医学院,海南海口571199 [7]天津大学应急医学研究院,天津300072 [8]海南医学院基础医学与生命科学学院

出　　处：《中华全科医学》2024年第6期926-930,935,共6页Chinese Journal of General Practice

基　　金：国家自然科学基金项目(81960420);海南省自然科学基金高层次人才项目(2019RC222);海南省重点研发项目(ZDYF2022SHFZ306)。

摘　　要：目的本研究旨在建立力竭运动引起的运动性心肌损伤小鼠模型,通过观察急性力竭运动后小鼠心肌损伤指标的变化,探讨益智仁预处理对力竭运动后心肌损伤的干预作用及其可能的机制。方法采用简单随机分组将小鼠分为5个实验组:对照组(Con,n=6)、益智仁组(Yz,n=6)、力竭组(EE,n=6)、力竭运动模型+益智仁低剂量组(EE+EYz,n=6)以及力竭运动模型+益智仁高剂量组(EE+HYz,n=6),所有小鼠均接受为期6周的特定运动干预与药物喂养,随后处死并收集小鼠心肌损伤相关生物标志物数据。结果在力竭次数方面,EE+HYz组和EE+EYz组相较于EE组差异有统计学意义(P<0.05)。在体重变化方面,EE+HYz组、Con组与Yz组,以及EE+HYz组与EE组比较差异均有统计学意义(P<0.05)。EE+HYz组的TNF-α水平与EE组相比显著降低(P<0.05);5组心肌型肌红蛋白(MYO)比较差异有统计学意义(P<0.05);其他4组与EE组心肌型肌酸激酶(CK-MB)比较差异有统计学意义(P<0.001)。结论益智仁具有显著的抗疲劳、抗炎症及对心肌损伤的保护作用。通过抑制力竭运动引起的心肌CK-MB和MYO活性的升高,益智仁降低了心肌损伤的程度。这些发现揭露了益智仁在心脏保护方面的潜在抗氧化作用,并可能成为预防和治疗运动相关心肌损伤的一个重要机制。此外,这些结果为开发心肌损伤干预和治疗药物提供了新的研究思路。Objective To establish a mouse model of exercise-induced myocardial injury caused by exhaustive exercise and to investigate the intervention effect and possible mechanism of Alpiniae oxyphylla fructus pretreatment on myocardial injury after exhaustive exercise by observing the changes of myocardial injury indexes after acute exhaustive exercise in mice,thus providing a theoretical basis and reference for further related studies.Methods Mice were randomly assigned to five different experimental groups by simple random grouping:Control group(Con,n=6),Yizhiren group(Yz,n=6),Exhaustion group(EE,n=6),Exhaustion exercise model+Yizhiren low dose group(EE+EYz,n=6),and Exhaustion exercise model+Yizhiren high dose group(EE+HYz,n=6),all mice received specific exercise intervention and drug feeding for six weeks.The mice were sacrificed at the end of the experiment,and data on biomarkers associated with myocardial injury were collected.Results In terms of exhaustion frequency,EE+HYz group and EE+EYz group showed statistically significant difference compared with EE group(P<0.05).Statistically significant differences were observed between EE+HYz group,Con and Yz group,EE+HYz and EE group(P<0.05).In the analysis of biochemical parameters,the level of tumor necrosis factorα(TNF-α)in the EE+HYz group was significantly decreased compared with that in the EE group(P<0.05).There was significant difference in erythroprotein(MYO)among the 5 groups(P<0.05).Myocardial creatine kinase(CK-MB)was significantly different between the other 4 groups and EE group(P<0.001).Conclusions Alpiniae oxyphylla fructus has significant anti-fatigue,anti-inflammation and protective effects on myocardial injury.By inhibiting the increase of myocardial CK-MB and MYO activity induced by exhaustive exercise,Alpiniae oxyphylla fructus reduced the degree of myocardial injury.These findings reveal the potential antioxidant effects of Alpiniae oxyphylla fructus in heart protection and may be an important strategy for the prevention and treatment of exercise-r

关 键 词：益智仁 力竭运动 氧化应激 心肌损伤 作用与机制 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]