大黄附子汤灌胃对小鼠重症急性胰腺炎的治疗作用及其机制  

Therapeutic effect and mechanism of Dahuang Fuzi decoction on severe acute pancreatitis in mice

作　　者：孙礼涛 路晓光[1] SUN Litao;LU Xiaoguang(Emergency Department,Zhongshan Hospital Affiliated to Dalian University,Dalian 116001,China)

机构地区：[1]大连大学附属中山医院急诊科,辽宁大连116001

出　　处：《山东医药》2024年第17期33-37,共5页Shandong Medical Journal

基　　金：国家自然科学基金项目(81673801)。

摘　　要：目的通过建立小鼠重症急性胰腺炎(SAP)模型,观察大黄附子汤对小鼠SAP的治疗作用并分析其机制。方法SPF小鼠按随机数字表法分为对照组、模型组、大黄附子汤组,每组8只。除对照组外,各组均通过腹腔注射左旋精氨酸建立大鼠SAP模型。大黄附子汤组在建模后6、12 h给予1.5 mL大黄附子汤灌胃,对照组、模型组在同时点给予1.5 mL生理盐水灌胃。末次灌胃结束后麻醉小鼠,采集眼球血,ELISA法检测SAP标志物淀粉酶(AMS)、脂肪酶(LPS)及炎症反应指标肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)。采集小鼠胰腺、肺、结肠组织样本,HE染色进行病理观察,对各组织进行病理学评分。采用免疫组织化学检测及Western blotting检测小肠组织α防御素6(DEFA6)、葡萄糖调节蛋白78(GRP78)蛋白,免疫荧光染色检测小肠组织溶菌酶。结果血清AMS、LPS、TNF-α、IL-6水平模型组>大黄附子汤组>对照组(P均<0.05)。对照组胰腺、肺、结肠组织结构完整,未见炎症细胞浸润;模型组胰腺、肺、结肠组织结构被破坏,可见红细胞渗出及炎症细胞浸润;大黄附子汤组各组织病理均较模型组有所改善;胰腺、肺、结肠组织病理学评分模型组>大黄附子汤组>对照组(P均<0.05)。小肠组织DEFA6蛋白表达模型组<大黄附子汤组<对照组,GRP78蛋白表达模型组>大黄附子汤组>对照组,溶菌酶相对荧光强度模型组<大黄附子汤组<对照组(P均<0.05)。结论大黄附子汤灌胃对小鼠SAP具有一定的治疗作用,其机制可能与抑制潘氏细胞内质网应激、增加抗菌分子DEFA6分泌从而改善肠屏障功能有关。Objective To explore the therapeutic effects of Dahuang Fuzi decoction(DHFZT)on severe acute pancreatitis(SAP)in mice through the establishment of mouse model with SAP.Methods The SPF mice were randomly divided into three groups:control group,model group,and DHFZT group,with 8 mice in each group.Except for the control group,the rat SAP models were established in each group by intraperitoneal injection of L-arginine.Mice in the the DHFZT group received oral administration of 1.5 mL DHFZT at 6 and 12 h after modeling,while mice in the control and model groups received oral administration of 1.5 mL normal saline.Following the final oral administration,anesthesia was administered to collect eye samples from all mice for ELISA detection of SAP markers such as amylase(AMS)and lipase(LPS),as well as inflammatory response markers including tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).Tissue samples from pancreas,lungs,and colon were collected for histological observation and calculation of histological scores.Immunohistochemistry and Western blotting were used to detectα-defensin 6(DEFA6)protein and glucose regulator protein 78(GRP78)in small intestine tissues,respectively;immunofluorescence staining was performed to detect lysozyme in the small intestine tissues.Results Serum AMS,LPS,TNF-α,IL-6 level were as follows:model group>DHFZT group>control group(all P<0.05).In the control group,the tissue structures of pancreas,lung and colon were intact,and no inflammatory cell infiltration was observed.In the model group,the tissue structures of pancreas,lung and colon were destroyed,and red blood cell exudation and inflammatory cell infiltration were observed.The histopathology of DHFZT group was improved compared with that of the model group.The histopathological scores of pancreas,lung and colon were as follows:model group>DHFZT group>control group(all P<0.05).Small intestine DEFA6 protein expression was as follows:model group<DHFZT group<control group,GRP78 protein expression was in the following order:model g

关 键 词：大黄附子汤 重症急性胰腺炎 潘氏细胞 α防御素 肠屏障功能 内质网应激 

分 类 号：R285.5[医药卫生—中药学]