傣药黑皮跌打乙酸乙酯萃取物的抗炎镇痛作用及急性毒性观察  

Anti-inflammatory and antinociceptive effects and acute toxicity of the ethyl acetate fraction of Fissistigma polyanthum

作　　者：藤丹 王祖慧 洪清莹 刘帅 蒋华夷 杨锐 何红平 TEND Dan;WANG Zuhui;HONG Qingying;LIU Shuai;JIANG Huayi;YANG Rui;HE Hongping(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院云南省南药可持续利用重点实验室,昆明650500

出　　处：《山东医药》2024年第17期38-42,47,共6页Shandong Medical Journal

基　　金：云南省科技厅中医联合专项面上项目(202301AZ070001-027);云南省科技人才和平台计划项目(202105AG070012)。

摘　　要：目的观察傣药黑皮跌打90%乙醇提取物(FPEE)乙酸乙酯萃取部位(FPEA)的抗炎镇痛作用及急性毒性。方法抗炎作用观察:ICR小鼠随机分为空白组、模型组、阳性对照组及FPEA低、中、高剂量组,阳性对照组给予地塞米松灌胃,FPEA低、中、高剂量组分别给予FPEA 200、400、800 mg/kg灌胃,空白组、模型组给予等体积生理盐水灌胃,持续给药5 d。除空白组外,各组均通过右后足足跖皮下注射角叉菜胶水溶液致炎,于致炎后1、2、3、4、5、6 h测定各组小鼠右后足足容积,计算足肿胀度及足肿胀抑制率;ELISA法检测致炎6 h后小鼠血清白细胞介素1β(IL-1β)、IL-6及肿瘤坏死因子α(TNF-α)。ICR小鼠随机分为模型组、阳性对照组及FPEA低、中、高剂量组,给药方法同上。采用二甲苯诱导小鼠耳肿胀,计算小鼠耳肿胀度及耳肿胀抑制率。镇痛作用观察:ICR小鼠随机分为模型组、阳性对照组及FPEA低、中、高剂量组,除阳性对照药选用阿司匹林及元胡止痛片外,各组给药方式同上。腹腔注射冰醋酸观察小鼠30 min内扭体次数并计算疼痛抑制率;于末次给药后30、60、90、120 min,将小鼠置于恒温热板上,记录小鼠痛阈值。急性毒性观察:ICR小鼠随机分为空白组、FPEE组、FPEA组,雌雄各半。FPEE组、FPEA组分别采用2000 mg/kg FPEE、FPEA灌胃,空白组给予等体积生理盐水灌胃。记录小鼠14 d内体质量,观察结束后处死小鼠,计算心脏、肝脏、脾脏、肺、肾脏及胸腺器官指数。结果与模型组比较,阳性对照组致炎4、5、6 h后足肿胀度减轻,FPEA中、高剂量组致炎5、6 h后足肿胀度减轻(P均<0.05)。致炎6 h后,FPEA高剂量与阳性对照组肿胀抑制率比较差异无统计学意义(P>0.05)。模型组血清IL-1β、IL-6及TNF-α水平均高于空白组,阳性对照组及FPEA低、中、高剂量组血清IL-6、TNF-α水平低于模型组,阳性对照组及FPEA中、高剂量组血清IL-1β水平低�Objective To investigate the anti-inflammatory and antinociceptive effects and acute toxicity of the ethyl acetate fraction(FPEA)from the 95%ethanol extraction of Fissistigma polyanthum(FPEE)in vivo.Methods Observation of anti-inflammatory effects:ICR mice were randomly divided into the blank control group,model group,positive group,and low-,medium-,and high-dose FPEA groups.Mice in low-,medium-,and high-dose FPEA groups were gavaged with 200,400,and 800 mg/kg of FPEA,respectively.Mice in the positive group were gavaged with dexamethasone,while mice in the blank control group and model group were gavaged with equal volume of normal saline.Mice were treated daily for five consecutive days.Except for the blank control group,the right hind paw of each mouse was subcutane ously injected with carrageenan to induce edema.The paw volume was measured at 1,2,3,4,5,and 6 h after the injection,then the edema degree and the inhibition rate of paw edema were calculated.The levels interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)in serum of the mice at 6 h after the injection were measured by ELISA.ICR mice were randomly divided into model group,positive group,and low-,medium-,and high-dose FPEA groups,and mice in each group were treated with the same methods as above.The ear edema degree and the inhibition rate of ear edema were calculated using the xylene-induced ear edema model.Observation of antinociceptive effects:ICR mice were randomly divided into the model group,positive group,and low-,medium-,and high-dose FPEA groups.Mice in each group were treated with the same methods as described above,but mice in the positive group were treated with the aspirin and Yuanhu Zhitong tablets.Each mouse was given an intraperitoneal injection of acetic acid,then the number of abdominal writhing was recorded for 30 min and the inhibition rate of writhing was calculated.The latency time was determined at 30,60,90,and 120 min after the drug administration.Observation of acute toxicity:ICR mice were randomly divided into th

关 键 词：黑皮跌打 乙酸乙酯部位 抗炎 镇痛 急性毒性 

分 类 号：R284[医药卫生—中药学]