加味过敏煎对Ⅰ型超敏反应的药效及其作用机制的研究  

Study on pharmacodynamics and mechanism of Jiawei Guomin Decoction on type I hypersensitivity

作　　者：韦义 钟海森 黄宝萱 张颖琳 覃骊兰[1] WEI Yi;ZHONG Hai-sen;HUANG Bao-xuan;ZHANG Ying-lin;QIN Li-lan(Guangxi University of Chinese Medicine,Nanning Guangxi,530001 China)

机构地区：[1]广西中医药大学,广西南宁530001

出　　处：《时珍国医国药》2024年第3期518-521,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81560755);广西中药药效研究重点实验室(19-050-39-A8);“桂派杏林拔尖人才”项目(04B22058C6)。

摘　　要：目的 研究加味过敏煎调控Ⅰ型超敏反应大鼠药效及作用机制,以明确加味过敏煎治疗Ⅰ型超敏反应免疫分子机制及作用靶点。方法 通过卵白蛋白(OVA)建立Ⅰ型超敏反应大鼠模型。将造模成功的动物分为模型组、地塞米松阳性对照组(8.4×10^(-5) g/kg)、加味过敏煎高、中、低剂量组(25.44 g/kg、12.72 g/kg、6.36 g/kg)。另设不造模的空白组。末次给药后,测定大鼠血清中免疫球蛋白E(IgE)、组胺、白介素-4(interleukin-4,IL-4)、肿瘤坏死因子(tumor necrosis factor-α,TNF-α)的因子含量;RT-PCR法测定大鼠脾脏中IFN-γ、T细胞中T-box(T-box expressed in T cells, T-bet)、IL-4、GATA结合蛋白3(GATA family of transcription factors3, GATA3)、白细胞介素-17(interleukin-17,IL-17)、白细胞介素-10 (interleukin-10,IL-10)、叉头状转录因子P3(forkhead box protein-3, FOXP3)的mRNA表达水平。结果 与模型组比较,加味过敏煎高、中剂量组明显降低Ⅰ型超敏反应大鼠血清中IgE(P<0.01,P<0.05)、组胺含量(P<0.05),升高IFN-γ水平(P<0.05),并且高剂量组能有效降低血清中IL-4水平(P<0.05);RT-PCR结果显示,与模型组比较,加味过敏煎高、中、低剂量组可以上调I型超敏反应大鼠脾组织T-bet(P<0.05,P<0.01)、IL-10(P<0.05)、FOXP3(P<0.05) mRNA表达,高、中剂量组显著促进IFN-γ mRNA表达(P<0.05)。同时加味过敏煎高、中、低剂量组下调IL-17mRNA表达(P<0.05),高剂量组显著抑制IL-4、GATA3 mRNA表达(P<0.05)。结论 加味过敏煎可以通过调节Th1/Th2细胞、Th17/Treg细胞平衡抑制炎症反应,进而影响免疫功能,达到抑制Ⅰ型超敏反应的作用。Objective Study the efficacy and mechanism of Jiawei Guomin decoction in regulating type I hypersensitivity Immune molecular mechanism and target of hypersensitivity. Methods To establish the mode of type I hypersensitivity by cvalban in(OVA) Animals that will be successfully modeled that divided into model group, dexamethasone positive control group(8.4×10^(-5) g/kg), high, medium and low dose group(25.44 g/kg, 12.72 g/kg, 6.36 g/kg) of Jiawei Guomin Decoction. Set up a blank group without modeling.After the last administration, then measured IgE, histamine, IL-4, IFN-γ of rat serum. The mRNA expression of IFN-γ, T-bet, IL-4, GATA3, IL-17, IL-10, Foxp3 in the spleen of was determined by the RT-PCR. Results Compared with the model group, the serum IgE(P<0.01,P<0.05) and histamine contents(P<0.05) of type I hypersensitivity rats were significantly reduced in the high and medium dose groups of Jiawei Guomin Decoction, and the IFN-γ level was increased(P<0.05). Moreover, the IL-4 level in the serum was effectively reduced in the high dose group(P<0.05). RT-PCR results showed that compared with the model group, Jiawei Guomin Decoction high, medium and low dose groups could up-regulate the expression of T-bet(P<0.05,P<0.01), IL-10(P<0.05) and FOXP3(P<0.05) mRNA in spleen tissue of type I hypersensitivity rats, and high and medium dose groups significantly promoted the expression of IFN-γ mRNA(P<0.05). At the same time Jiawei Guominjian high, medium and low dose group down-regulated IL-17 mRNA expression(P<0.05), high dose group significantly inhibited IL-4, GATA3 mRNA expression(P<0.05).Conclusion Jiawei Guomin Decoction can inhibit inflammatory response by regulating the balance of Th1/Th2 cells and Th17/Treg cells, thereby affecting immune function and inhibiting type I hypersensitivity.

关 键 词：加味过敏煎 Ⅰ型超敏反应 药效研究 机制研究 

分 类 号：R285.5[医药卫生—中药学]