Cardioprotective Potential of Cymbopogon citratus Essential Oil against Isoproterenol-induced Cardiomyocyte Hypertrophy:Possible Involvement of NLRP3 Inflammasome and Oxidative Phosphorylation Complex Subunits  

作　　者：Xiao-yun DING Hao ZHANG Yu-mei QIU Meng-die XIE Hu WANG Zheng-yu XIONG Ting-ting LI Chun-ni HE Wei DONG Xi-lan TANG 

机构地区：[1]School of Pharmacy,Jiangxi Science&Technology Normal University,Nanchang 330013,China [2]Key Laboratory of Modern Preparation of Chinese Medicine,Ministry of Education,Jiangxi University of Chinese Medicine,Nanchang 330004,China [3]Jiangxi Provincial Key Laboratory of Drug Design and Evaluation,Nanchang 330013,China

出　　处：《Current Medical Science》2024年第2期450-461,共12页当代医学科学（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(Nos.81960732 and 82060733);the Natural Science Foundation of Jiangxi Province(No.20224BAB206111);the Science and Technology Plan of Jiangxi Provincial Health Commission(No.202311141);the Open Project of Jiangxi Provincial Key Laboratory of Drug Design and Evaluation(No.JKLDE-KF-2101);the Open Project of Key Laboratory of Modern Preparation of TCM,Ministry of Education,Jiangxi University of Chinese Medicine(No.TCM-201911).

摘　　要：Objective:Cymbopogon citratus(DC.)Stapf is a medicinal and edible herb that is widely used for the treatment of gastric,nervous and hypertensive disorders.In this study,we investigated the cardioprotective effects and mechanisms of the essential oil,the main active ingredient of Cymbopogon citratus,on isoproterenol(ISO)-induced cardiomyocyte hypertrophy.Methods:The compositions of Cymbopogon citratus essential oil(CCEO)were determined by gas chromatography-mass spectrometry.Cardiomyocytes were pretreated with 16.9µg/L CCEO for 1 h followed by 10µmol/L ISO for 24 h.Cardiac hypertrophy-related indicators and NLRP3 inflammasome expression were evaluated.Subsequently,transcriptome sequencing(RNA-seq)and target verification were used to further explore the underlying mechanism.Results:Our results showed that the CCEO mainly included citronellal(45.66%),geraniol(23.32%),and citronellol(10.37%).CCEO inhibited ISO-induced increases in cell surface area and protein content,as well as the upregulation of fetal gene expression.Moreover,CCEO inhibited ISO-induced NLRP3 inflammasome expression,as evidenced by decreased lactate dehydrogenase content and downregulated mRNA levels of NLRP3,ASC,CASP1,GSDMD,and IL-1β,as well as reduced protein levels of NLRP3,ASC,pro-caspase-1,caspase-1(p20),GSDMD-FL,GSDMD-N,and pro-IL-1β.The RNA-seq results showed that CCEO inhibited the increase in the mRNA levels of 26 oxidative phosphorylation complex subunits in ISO-treated cardiomyocytes.Our further experiments confirmed that CCEO suppressed ISO-induced upregulation of mt-Nd1,Sdhd,mt-Cytb,Uqcrq,and mt-Atp6 but had no obvious effects on mt-Col expression.Conclusion:CCEO inhibits ISO-induced cardiomyocyte hypertrophy through the suppression of NLRP3 inflammasome expression and the regulation of several oxidative phosphorylation complex subunits.

关 键 词：Cymbopogon citratus essential oil cardiac hypertrophy NLRP3 inflammasome oxidative phosphorylation complex subunits 

分 类 号：R692[医药卫生—泌尿科学]