2021-2022年华东地区猪繁殖与呼吸综合征病毒的分子流行病学调查  

Molecular epidemiological survey of porcine reproductive and respiratory syndrome virus in East China from 2021 to 2022

作　　者：张杰 张路捷 周昊然 刘星 白娟 姜平 ZHANG Jie;ZHANG Lujie;ZHOU Haoran;LIU Xing;BAI Juan;JIANG Ping(Key Laboratory of Animal Bacteriology,Ministry of Agriculture/College of Veterinary Medicine,Nanjing Agricultural University,Nanjing210095,China)

机构地区：[1]南京农业大学,动物医学院,农业部动物细菌学重点实验室,江苏南京210095

出　　处：《中国兽医科学》2024年第4期519-525,共7页Chinese Veterinary Science

基　　金：江苏省农业科技自主创新资金项目(CX(22)1011);国家生猪产业技术体系专项(CARS-36);国家自然科学基金项目(32230103)。

摘　　要：为初步了解我国华东地区猪繁殖与呼吸综合征病毒(PRRSV)的流行特点,2021-2022年采集华东地区480份临床疑似PRRSV样品,采用RT-PCR方法检测是否存在PRRSV,统计显示阳性为175份,阳性检出率为38.89%。对来自不同猪场的24个阳性样品的病毒ORF5基因测序,所有检测的毒株均属美洲型,共可以分为4个亚型,其中11个毒株属于高致病性PRRSV(HP-PRRSV)(Sublineage 8.7);6个毒株属于类NADC30毒株亚型(Sublineage 1.8);4个毒株属于类NADC34亚型(Sublineage 1.5)。病毒GP5蛋白基因测序结果显示,流行毒株GP5蛋白aa3-39及aa185-200两个高变区出现变异。该研究表明我国该地区PRRSV流行毒株存在遗传多样性,基因型复杂,需要引起我们的重视。In order to preliminarily study the epidemic characteristics of porcine reproductive and respiratory syndrome virus(PRRSV)in East China,480 clinically suspected PRRSV-positive samples were collected in East China from 2021 to 2022 for PRRSV detection by RT-PCR.Of these samples,175 were PRRSV-positive with a positive rate of 38.89%.The ORF5 genes of 24 positive samples from different pig farms were sequenced and shown to be the American type 2,divided into 4 subtypes.Among them,ll strains belonged to the HP-PRRSV(Sublineage 8.7),6 strains belonged to the PRRSV NADC30-like strain(Sublineage 1.8),and 4 strains belonged to the PRRSV NADC34-like strain(Sublineage 1.5).The PRRSV GP5 sequencing results showed that mutations occurred in the two hypervariable regions aa3-39 and aa185-200 of the epidemic strains.This study shows that the circulating PRRSV strains in East China have a high genetic diversity with complex genotypes,which calls for our special attention.

关 键 词：猪繁殖与呼吸综合征病毒 ORF5 NADC34 NADC30 遗传变异 

分 类 号：S852.659.6[农业科学—基础兽医学]