叶尔羌河流域鲁道夫对盲囊线虫的分子鉴定及遗传多样性分析  

Molecular identification and genetic diversity analysis of Contracaecum rudolphii in the Yarkand River basin

作　　者：王瑾璞 刘彦君 石彩霞 魏念文 张丽 岳城[1] 郝翠兰[1] WANG Jinpu;LIU Yanjun;SHI Caixia;WEI Nianwen;ZHANG Li;YUE Cheng;HAO Cuilan(College of Veterinary Medicine,Xinjiang A gricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《中国兽医科学》2024年第4期526-532,共7页Chinese Veterinary Science

基　　金：新疆维吾尔自治区自然科学基金项目(No.2021D01B56)。

摘　　要：为评估叶尔羌河流域塔里木裂腹鱼体内寄生的鲁道夫对盲囊线虫(Contracaecum rudolphii)群体遗传多样性水平,本研究采用PCR方法测定叶尔羌河、塔什库尔干河、下坂地水库3个地区52条对盲囊线虫的ITS-1和COⅠ基因序列,对其基因序列及遗传结构进行鉴别分析。结果显示,基于ITS-1序列叶尔羌河流域塔里木裂腹鱼体内对盲囊线虫被鉴定为鲁道夫对盲囊线虫B种(C.rudolphii B),利用COⅠ序列共检测出30个单倍型,其中共享单倍型1种。多样性分析显示,3个群体的整体单倍型多样性(Hd)和核苷酸多样性(Pi)分别为0.931和0.00967。系统进化树和单倍型网络结构表明,3个群体间单倍型随机地聚集在一起,没有形成明显的空间地理格局。群体遗传结构和分子方差分析表明,3个群体间遗传距离较小且呈弱分化水平,鲁道夫对盲囊线虫B种的分化主要来自于群体内部。研究结果表明,叶尔羌河流域3个不同地理群体的鲁道夫对盲囊线虫B种均属高遗传多样性群体,各群体间基因交流频繁,没有形成特定的地理遗传结构,正处于稳定扩增状态,本研究旨在为保护其宿主塔里木裂腹鱼种质资源提供理论依据。In order to assess the genetic diversity level of Contracaecum rudolphii population parasitising Schizothorax biddulphi in the Yarkant River Basin,the sequences of ITS-l and Co I genes of 52 Contracaecums from three regions,namely,the Yarkant River,the Tashkurgan River and the Xiabandi Reservoir,were determined by PCR,and the gene sequences and their genetic structure were analysed.The results showed that Contracaecum in Schizothorax biddulphi in the Yarkand River Basin were all identified as C.rudolphii B based on ITS-1 genes,and a total of 30 haplotypes were detected based on CO I genes,including one shared haplotype.Genetic diversity analysis showed that the overall haplotype diversity(Hd)and nucleotide diversity(Pi)of the three populations were 0.931 and 0.00967,respectively.Phylogenetic trees and haplotype network structures indicate that haplotypes clustered randomly among the three populations and did not form a distinct geographical distribution.Population genetic structure and AMOVA analysis showed that the genetic distances among the 3 populations were small and at a weak differentiation level,and the differentiation of C.rudolphii B was mainly from within the populations.The conclusions showed that the populations of C.rudolphii B from 3 different geographical groups in the Yarkant River Basin were all high genetic diversity populations,with frequent gene exchanges among the populations,which did not form a specific geographic genetic structure,and were in a state of stable amplification,and that the present study was aimed at providing theoretical basis for the conservation of Schizothorax biddulphi.

关 键 词：鲁道夫对盲囊线虫 COⅠ基因 ITS-1基因 遗传多样性 

分 类 号：S852.731[农业科学—基础兽医学]