纳米三氧化二铁经口暴露对雄性大鼠生殖系统的影响  

Effects of oral exposure to iron oxide nanoparticles on reproductive system of male rats

作　　者：赵靖 苏欣怡 侍江春 谢羽檬 罗雅亭 王雪 李云[1] Zhao Jing;Su Xinyi;Shi Jiangchun;Xie Yumeng;Luo Yating;Wang Xue;Li Yun(West China School of Public Health/West China Fourth Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,成都610041

出　　处：《卫生研究》2024年第3期435-440,共6页Journal of Hygiene Research

基　　金：国家重点研发计划(No.2017YFC1600204)。

摘　　要：目的探讨纳米三氧化二铁颗粒(iron oxide nanoparticles,Fe_(2)O_(3)NPs)经口暴露对雄性大鼠生殖系统的影响。方法将40只雄性SD大鼠按体重随机分配到对照组和低、中、高剂量干预组,每组10只,分别灌胃生理盐水和50、100、200 mg/kg的Fe_(2)O_(3)NPs混悬液,每次灌胃体积10 mL/kg,连续灌胃28 d,每3天称一次体重,记录大鼠体重变化。10%水合氯醛腹腔麻醉后,脱颈处死大鼠,收集睾丸和附睾。称重并计算睾丸系数和附睾系数,苏木精-伊红染色观察大鼠睾丸组织病理学切片,光学显微镜下统计附睾精子数目、计算精子畸形率,试剂盒法检测大鼠睾丸组织匀浆中组织标志酶酸性磷酸酶、碱性磷酸酶、乳酸脱氢酶和γ-谷氨酰转肽酶活性,以及氧化应激指标超氧化物歧化酶活性和谷胱甘肽、丙二醛含量。结果与对照组相比,各剂量Fe_(2)O_(3)NPs干预组大鼠体重、睾丸系数及附睾系数差异无统计学意义;100和200 mg/kg组生精上皮排列紊乱、生精细胞减少;200 mg/kg组精子数目减少,100和200 mg/kg组精子畸形率增加(P<0.01);100和200 mg/kg组酸性磷酸酶活性升高(P<0.05)、γ-谷氨酰转肽酶活性降低(P<0.01);100 mg/kg组谷胱甘肽水平升高(P<0.05),100和200 mg/kg组丙二醛含量升高(P<0.01)。结论Fe_(2)O_(3)NPs可引起大鼠睾丸组织结构损伤,精子数目减少,精子畸形率增高,干扰睾丸组织标志酶活性并诱导氧化应激,对雄性大鼠生殖系统造成了不利影响。OBJECTIVE To investigate the effects of oral exposure to iron oxide nanoparticles(Fe_(2)O_(3)NPs)on the reproductive system of male rats.METHODS Forty male SD rats were randomly divided into control group and low,medium,high dose groups,10 rats in each group,normal saline and 50,100 and 200 mg/kg Fe_(2)O_(3)NPs suspension were given by gavage,respectively.The volume of gavage was 10 mL/kg for 28 days.The body weight was weighed every three days,and the body weight changes of rats were recorded.After intraperitoneal anesthesia with 10%chloral hydrate,the rats were sacrificed by cervical dislocation,and the testis and epididymis were collected.Weigh and calculate the testicular coefficient and epididymal coefficient,the pathological sections of rat testis were observed by hematoxylin-eosin staining,the number of epididymal sperm was counted under an optical microscope and the sperm deformity rate was calculated.The activities of acid phosphatase(ACP),alkaline phosphatase(AKP),lactate dehydrogenase(LDH)andγ-glutamyl transpeptidase(γ-GT),the activity of superoxide dismutase(SOD),and the contents of glutathione(GSH)and malondialdehyde(MDA)in rat testis homogenate were detected by kit method.RESULTS Compared with control group,there was no significant difference in body weight,testicular coefficient and epididymal coefficient in each dose group.In the medium and high dose groups,the arrangement of spermatogenic epithelium was disordered and spermatogenic cells decreased.The number of sperm in high dose group was decreased,and the sperm deformity rate in medium and high dose groups was increased(P<0.01).The activity of ACP in medium and high dose groups increased(P<0.05),and the activity ofγ-GT decreased(P<0.01).There was no significant change in the activity of AKP and LDH in testicular homogenate of rats in each group(P>0.05).The level of GSH in medium dose group was increased(P<0.05),and the content of MDA in medium and high dose groups was increased(P<0.01).There was no significant difference in SOD activity amon

关 键 词：纳米氧化铁 消化道暴露 生殖毒性 

分 类 号：R114[医药卫生—卫生毒理学]