额尔敦-乌日勒对β-淀粉样蛋白_(25~35)所致阿尔茨海默病模型大鼠脑内神经元凋亡的影响  

Effects of Erden-Ujile on Neuronal Apoptosis Induced by β-amyloid_(25~35) in the Brain of Rats with Alzheimer's Disease

作　　者：叶芸 李士伟 白塔娜 李怀楠 乔鹏 毛环宇 张小玲 徐鹏 YE Yun;LI Shi-Wei;BAI Ta-Na;LI Huai-Nan;QIAO Peng;MAO Huan-Yu;ZHANG Xiao-Ling;XU Peng(Department of Neurology,Tongliao Hospital,Tongliao 028000,China;Hohhot International Travel Healthcare Center(Out-Patient Department of Hohhot Customs Port),Hohhot 010020,China)

机构地区：[1]通辽市医院神经内三科,内蒙古通辽028000 [2]呼和浩特国际旅行卫生保健中心(呼和浩特海关口岸门诊部),呼和浩特010020

出　　处：《中国药物经济学》2024年第4期109-112,118,共5页China Journal of Pharmaceutical Economics

基　　金：内蒙古自治区卫生健康委医疗卫生科技计划项目(202201572)。

摘　　要：目的探讨额尔敦-乌日勒对β-淀粉样蛋白(Aβ)_(25~35)所致阿尔茨海默病模型大鼠脑内神经元凋亡的影响。方法将Aβ_(25~35)所致阿尔茨海默病模型大鼠(n=54)随机分为3组:模型组、低剂量组、高剂量组。3组分别给予等体积的0.9%氯化钠注射液、50μg/ml额尔敦-乌日勒、100μg/ml额尔敦-乌日勒灌胃,1次/d,连续灌胃14 d。比较3组大鼠治疗不同时间点水迷宫逃避潜伏期,血清超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,Caspase3蛋白表达水平。结果治疗7 d、14 d,低剂量组、高剂量组大鼠水迷宫逃避潜伏期短于模型组(P<0.05),且高剂量组短于低剂量组(P<0.05);治疗7 d、14 d,低剂量组、高剂量组大鼠血清SOD水平高于模型组(P<0.05),血清MDA水平低于模型组(P<0.05),且高剂量组SOD、MDA水平变化幅度优于低剂量组(P<0.05);治疗7 d、14 d,低剂量组、高剂量组大鼠血清TNF-α、IL-6水平低于模型组(P<0.05),且高剂量组低于低剂量组(P<0.05);治疗7 d、14 d,低剂量组、高剂量组神经元组织的Caspase3蛋白相对表达水平低于模型组(P<0.05),且高剂量组低于低剂量组(P<0.05)。结论额尔敦-乌日勒在Aβ_(25~35)所致阿尔茨海默病模型大鼠的应用能缩短水迷宫逃避潜伏期,提高血清SOD水平,降低血清TNF-α、IL-6、MDA水平,还可抑制神经元细胞凋亡。Objective To explore the effect of Erden-Ujile on neuronal apoptosis induced byβ-amyloid protein(Aβ)_(25-35) in Alzheimer's disease model rats.Methods The model rats of Alzheimer's disease induced by Aβ_(25~35)(n=54)were randomly divided into three groups:model group,low dose group and high dose group.Three groups were given equal volume of saline,50μg/ml Erdun-Uzhle and 100μg/ml Erden-Ujile gavage once a day for 14 days,respectively.The levels of serum superoxide dismutase(SOD),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and the expression of Caspase3 protein were compared in 3 groups of rats treated with water maze at different time.ResultsAfter 7 and 14 days of treatment,the water maze escape latency of rats in low-dose and high-dose groups was shorter than that in model group(P<0.05),and that in high-dose group was shorter than that in low-dose group(P<0.05).After 7 and 14 days of treatment,the serum SOD level in low-dose and high-dose groups was higher than that in model group(P<0.05),and the serum MDA level was lower than that in model group(P<0.05),and the change range of SOD and MDA levels in high-dose group was better than that in low-dose group(P<0.05).After 7 and 14 days of treatment,the serum levels of TNF-αand IL-6 in low-dose and high-dose groups were lower than those in model group(P<0.05),and those in high-dose group were lower than those in low-dose group(P<0.05);After 7 and 14 days of treatment,the relative expression level of Caspase3 protein in neurons of low-dose and high-dose groups was lower than that of model group(P<0.05),and that of high-dose group was lower than that of low-dose group(P<0.05).Conclusion The application of Erden-Uhelle in Aβ_(25-35) induced Alzheimer's disease model rats can shorten the water maze escape latency,increase serum SOD level,decrease serum TNF-α,IL-6,MDA levels,and inhibit neuronal cell apoptosis.

关 键 词：额尔敦-乌日勒 β-淀粉样蛋白_(25~35) 阿尔茨海默病 大鼠 超氧化物歧化酶 神经元 细胞凋亡 

分 类 号：R592[医药卫生—老年医学] R285.5[医药卫生—内科学]