长工时与DNA加合物εdA、εdC水平表达相关性研究  

Correlation between long working hours and expression of DNA adducts εdA and εdC levels

作　　者：柳云龙 王瑾[1] 李海斌[1] 崔师伟[1] 王馨蒙 张思雨[1] 刘晓曼[1] 李霜[1] 孙新[1] LIU Yunlong;WANG Jin;LIHaibin;CUI Shiwei;WANG Xinmeng;ZHANG Siyu;LIU Xiaoman;LI Shuang;SUN Xin(National Institute of Occupational Health and Poisoning Control,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]杭州医学院,浙江杭州310053

出　　处：《工业卫生与职业病》2024年第3期201-207,共7页Industrial Health and Occupational Diseases

基　　金：“十四五”国家重点研发计划“职业伤害防控关键技术与干预策略研究”重点研发计划项目(2022YFC2503205)。

摘　　要：目的研究工作时长对护士外周血中Etheno-DNA加合物(εdA、εdC)水平的影响。方法本研究采用横断面研究方法,于2022-2024年期间募集来自广东省清远市、湖南省长沙市、新疆维吾尔自治区乌鲁木齐市以及重庆市等四个地区共12家医院的女性护士为研究对象。研究对象纳入标准:(1)身体健康,无基础疾病史;(2)在岗临床注册护士,且从事护理工作一年以上;(3)年龄不超过55岁;(4)研究对象无长期吸烟史与饮酒史;(5)月经周期正常;(6)研究对象同意参加本研究,并自愿签署知情同意。排除标准:(1)孕产妇;(2)有乳腺癌患病史,或有直系亲属(父母、子女及兄弟、姐妹)乳腺癌家族史;(3)临床诊断的糖尿病、高血压、高血脂、心血管系统疾病、其他类型肿瘤、肾脏疾病、慢性疼痛;(4)过去三个月内服用过含褪黑素的补剂、免疫抑制剂、激素类药物、降糖药物、降脂药物或类固醇药物、抗氧化补充剂;(5)ICU、放射科或急诊科;(6)长期病假者。通过问卷现场调查,收集研究对象工作时长、年龄、工龄、健康特征等基本情况。按照工作时间进行分组,包括标准工作时长组35~40 h/周25人,41~48小时/周36人、≥49小时/周30人的四个组,共纳入研究对象91人。采集每位研究对象的班前空腹静脉血5 ml,采用超高效液相色谱串联质谱(UPLC-MS-MS)方法检测研究对象外周血中Etheno-DNA加合物(εdA、εdC)水平。通过单因素分析探索年龄、工龄、健康特征对Etheno-DNA加合物(εdA、εdC)水平的影响;利用多元线性回归模型检验在平衡可能影响加合物水平的混杂因素后,护士工作时长与Etheno-DNA加合物(εdA、εdC)水平的关联性。结果与标准工作时长组(35~40 h/周)相比,≥49 h/周组研究对象血中εdA和εdC加合物水平显著升高(ZεdC=2.424,ZεdA=1.776;P<0.05);研究采取多元线性回归,在平衡了可能的混杂因素(年龄、工龄、健康特征等)后,发Objective To study the effect of working hours on Etheno-DNA adduct(εdA,εdC)levels in nurses peripheral blood.Methods In this study,a cross-sectional survey was used to recruit female nurses as the study subject from a total of 12 hospitals in four regions,including Qingyuan City in Guangdong Province,Changsha City in Hunan Province,Urumqi City in Xinjiang Uygur Autonomous Region,and Chongqing Municipality,from 2022 to 2024.Inclusion criteria were:(1)healthy,no history of underlying diseases;(2)on-the-job clinical registered nurses who have been engaged in nursing for more than one year;(3)not older than 55 years;(4)no history of long-term smoking and alcoho consumption;(5)normal menstrual cycle;(6)consent to participate in the study and sign the informed consent voluntarily Exclusion criteria were:(1)maternal;(2)history of breast cancer,or family history of breast cancer in the immediate family(parents,children,brothers and sisters);(3)clinically diagnosed diabetes mellitus,hypertension,hyperlipidemia,cardiovascular disease,other types of tumors,renal disease,chronic pain;(4)taking melatonin-containing supplements,immunosuppressants,hormonal drugs,hypoglycemic drugs,lipid-lowering drugs,or steroids in the past three months,lipid-lowering or steroid medications,and antioxidant supplements;(5)ICU,radiology,or emergency department;and(6)long-term sick leave.An on-site questionnaire was used to collect the basic information of the study participants,such as working hours,age,length of service,and health characteristics.Grouped according to working hours,including 25 people in the standard working hours group(35-40 h/week),36 people in the 41-48 h/week group,and 30 people in the≥49 h/week group in four groups,a total of 91 study subjects were included.Five ml of pre-shift fasting venous blood was collected from each study subject,and the levels of Etheno-DNA adducts(εdA,εdC)in the peripheral blood of the study subjects were detected by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS-MS)

关 键 词：长工时 氧化应激 脂质过氧化 Etheno-DNA加合物 

分 类 号：R12[医药卫生—环境卫生学]