楮头红水提物抗氧化活性及改善小鼠肝损伤的研究  

Antioxidant and Improvement of Liver Injury in Mice by Sarcopyramis Nepalensis Wall Aqqueous Extracts

作　　者：华宝玉[1,2] 陈森艳 郭昌 邱龙新[1,2] HUA Bao-yu;CHEN Seng-yan;GUO Chang;QIU Long-xin(School of Life Science,Longyan University,Longyan 364012,China;Fujian Provincial Universities Key Laboratory of Preventive Veterinary Medicine and Biotechnology,Longyan University,Longyan 364012,China)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]龙岩学院预防兽医学与生物技术福建省高校重点实验室,福建龙岩364012

出　　处：《嘉应学院学报》2024年第3期57-61,共5页Journal of Jiaying University

基　　金：福建省教育厅中青年教师教育科研项目(JAT210441)。

摘　　要：目的:研究楮头红水提物抗氧化活性及对急性酒精肝损伤小鼠的保护作用.方法:制备楮头红水提物,采用羟自由基和DPPH方法评价其体外抗氧化性;将体重均衡的昆明雄鼠,随机分成六组:空白组,酒精组,阳性组(联苯双酯),楮头红水提物低、中、高剂量组.建立急性酒精肝损伤模型后,制作切片进行HE染色观察肝组织病理变化,检测血清中AST、ALT活性,测定肝组织GSH含量、GSH-PX活性、SOD活性、MDA含量.结果:楮头红水提物对羟自由基和DPPH具有清除作用;病理检查显示水提物组的肝组织脂肪变性程度随着剂量的增加不断减小,被酒精损伤所致的症状明显改善;楮头红水提物能够显著改善被酒精诱导变化的一些抗氧化生化指标,即显著降低了小鼠体内AST和ALT活性,提高了肝组织的GSH、GSH-PX、SOD的水平,降低了MDA含量.结论:楮头红水提物可以通过提高肝脏抗氧化水平改善小鼠急性酒精性肝损伤.Objective:To study protective effect of aqqueous extracts from SarcopyramisNepalensisWall(SNE)on acute alcoholic liver injury in mice.Method:Prepare the SNE aqqueous extracts,and then hydroxyl free radicals and DPPH radicals methods were used to evaluate antioxidant activity.Kunming male mice were randomly divided into six groups,namely blank group,alcoholic group,positive control group with Bifendatatum,and high,medium,low dose of SNE groups respectively.Then blood was collected from eyeball for testing ALT activity and AST activity,liver tissue was dissected and modified into slice for HE staining,and liver tissues GSH content,GSH-PX activity,SOD activity and MDA content were assayed with kits.Result:The SNE aqqueous extracts possessed high efficiency to has scavenge hydroxyl free radicals and DPPH radicals.Compered with model group,the degree of hepatic steatosis decreased constantly with the increasing dose of SNE,and the alcohol-induced injuries were relieved.SNE aqqueous extracts significantly improve the anti-oxidative biochemical indexes stimulated by alcohol.The enzyme activity of AST and ALT in the body of mice were significantly decreased respectively by SNE treatment,the content of GSH,GSH-PX and SOD were increased respectively,and the content of MDA wase reduced.Conclusion:The SNE aqqueous extracts has improve the alcoholic liver injury in mice by strengthening the ability of anti-oxidation.

关 键 词：楮头红水提物 急性酒精肝损伤 抗氧化 

分 类 号：R284.2[医药卫生—中药学]