鳙BMP基因家族成员的鉴定、进化及表达分析  

GENOME WIDE IDENTIFICATION,PHYLOGENY,AND EXPRESSION OF BONE MORPHOGENETIC PROTEIN GENES IN BIGHEAD CARP(HYPOPHTHALMICHTHYS NOBILIS)

作　　者：武艳红 周小雨 陈庚 俞小牧[1] 童金苟[1,2] WU Yan-Hong;ZHOU Xiao-Yu;CHEN Geng;YU Xiao-Mu;TONG Jin-Gou(State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100039,China)

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2024年第7期1085-1094,共10页Acta Hydrobiologica Sinica

基　　金：中国科学院战略性科技先导专项(XDA24030505-1);国家自然科学基金(32072973)资助。

摘　　要：为了深入研究鳙(Hypophthalmichthys nobilis)骨形态发生蛋白(Bone morphogenetic protein,BMP)基因(Hyn-bmps)的结构、功能和表达特征,实验首先通过生物信息学方法对Hyn-bmps家族进行了全基因组水平的鉴定,利用qRT-PCR法检测其在不同组织和快/慢速生长差异个体中的表达模式。在鳙全基因组水平上共鉴定出不均匀地分布于13条染色体上的18个BMP家族成员。分子系统发育分析将BMP划分为5个亚家族;内含子-外显子结构和保守基序的分析结果不仅表明Hyn-bmps在进化上高度保守,也为鳙亚家族的分类提供了证据。18个bmps在鳙不同组织中具有不同的表达模式。群体中极端快/慢速生长差异个体的组织表达分析结果表明,绝大部分Hyn-bmps的高表达与快速生长呈高度正相关,提示它们在鳙机体生长和头部发育中具有正调控作用。研究结果不仅表明BMP基因家族在鳙生长与发育中扮演重要角色,而且也为鳙选择育种和分子育种研究提供了重要的基因资源。Bone morphogenetic protein(BMP)play a crucial role in regulating the skeletal development of fish,significant influencing external morphology,functional exercise and even survival rates in aquaculture breeding.Bighead carp(Hypophthalmichthys nobilis)is a species of particular interest due to the importance of its head and body shape.In this study,we identified a total of 18 BMP from the bighead carp genome,which are unevenly distributed on 13 chromosomes.BMP were divided into five sub-families based on phylogenetic analysis,revealing a high level of conservation among BMP.The expression of Hyn-bmps was investigated in a population exhibiting extremely fast/slow growth differences.Most Hyn-bmps revealed a positive correlation with growth,suggesting their potential positive regulatory effects on the growth and head development of bighead carp.These results not only indicate the significant role of the BMP genes family in the growth and development of bighead carp,but also provide valuable insights for molecular breeding efforts targeting this species.

关 键 词：BMP 基因家族 基因特征 基因表达 鳙 

分 类 号：Q344.1[生物学—遗传学]