一体式PN/A工艺处理污泥热水解厌氧消化液性能研究及微生物解析  

The performance and microbial community analysis of an one-stage PN/A process for treating anaerobic digestion effluent after thermal hydrolysis

作　　者：岳文慧 隋倩雯[1,2] 魏源送[1,2,3] 陈彦霖 王拓 YUE Wenhui;SUI Qianwen;WEI Yuansong;CHEN Yanlin;WANG Tuo(Laboratory of Water Pollution Control Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;State Key Joint Laboratory of Environment Simulation and Pollution Control,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;University of Chinese Academy of Sciences,Beijing 101408;Chongqing Innovation Center on Three Gorges Ecological&Environmental Technology,Chongqing 400000;China National Complete Plant Import&Export Group Corporation Ltd.,Beijing 100011)

机构地区：[1]中国科学院生态环境研究中心,水污染控制实验室,北京100085 [2]中国科学院生态环境研究中心,环境模拟与污染控制国家重点联合实验室,北京100085 [3]中国科学院大学,北京101408 [4]重庆市三峡生态环境技术创新中心,重庆400000 [5]中国成套设备进出口集团有限公司,北京100011

出　　处：《环境科学学报》2024年第6期1-10,共10页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划项目(No.2021YFC3200601);江西省重点研发计划项目(No.20223BBG71016);中国科学院青年创新促进会项目(No.2021042)。

摘　　要：污泥热水解厌氧消化液是一种典型的高氨氮、高COD且含有抑制性难降解有机物的废水.本研究采用一体式部分亚硝化-厌氧氨氧化(PN/A)工艺处理污泥热水解厌氧消化液,通过曝气模式优化调控实现了脱氮性能提升,并研究了长期稳定运行的可行性.结果表明,污泥热水解厌氧消化液对氨氧化菌(AOB)与厌氧氨氧化菌(AnAOB)活性均有抑制,且对AnAOB抑制更明显.调整间歇曝气模式且控制DO<0.5mg·L^(-1),可实现80.6%±3.1%总氮去除率,总氮负荷达到(0.069±0.028)kg·m^(-3)·d^(-1),但长期稳定运行有待优化.AOB的优势菌属为Nitrosomonas属,其相对丰度因污泥热水解厌氧消化液的引入先降低,后随曝气模式调整适应后恢复;AnAOB的优势菌属为Ca.Brocadia,其相对丰度随热水解消化液的持续引入而持续降低,对长期稳定运行提出挑战;反硝化菌(DNB)的优势菌属为Thauera,其相对丰度略升高,反硝化脱氮贡献增强.KEGG功能预测表明膜转运及信号传导通路丰度持续降低,影响群落菌属间膜运输途径的物质信息交流,可能是污泥热水解厌氧消化液抑制作用发生的机制.Sludge dewatering liquor from thermal hydrolysis pretreatment coupled anaerobic digestion(THP-AD)is a typical high-strength wastewater containing high ammonia and recalcitrant organic compounds.This study focused on the treatment of THP-AD dewatering liquor using one-stage partial-nitritation and anammox process(PN/A)within an activated sludge system.The enhancement of nitrogen removal was obtained through the optimization of aeration patterns,and stable long-term operation was investigated.The findings revealed that THP-AD dewatering liquor exhibited inhibitory effects on ammonia oxidation bacteria(AOB)and a more ponounced impact on anaerobic ammonia oxidation(Anammox)bacteria.Total nitrogen removal efficiency(NRE)of 80.6%±3.1%and TN removal rate(TNRR)of(0.069±0.028)kg·m^(-3)·d^(-1) were achieved under an intermittent aeration mode at dissolved oxygen(DO)concentration below 0.5 mg·L^(-1).However,the study identified the imperative improvement for its long-term stable operation.The dominant genus of AOB,Nitrosomonas,experienced a decline upon the introduction of THP-AD dewatering liquor,followed by recovery after the change of the aeration pattern.Conversely,the relative abundance of Ca.Brocadia,the predominant genus of anammox bacteria,exhibited a consistent decrease over the operational period,posing a challenge to long-term stability.The dominant genus of denitrifying bacteria(DNB)is Thauera,with a slightly increased abundance,enhancing the denitrification contribution for nitrogen removal.KEGG functional predictions indicated a continuous decrease in the abundance of membrane transport and signal transduction pathways,affecting the inter-genus membrane transport information exchange within the community.This may be a mechanism underlying the inhibitory effects of THP-AD dewatering liquor.

关 键 词：热水解 污泥厌氧消化液 厌氧氨氧化 控制模式 间歇曝气 

分 类 号：X703[环境科学与工程—环境工程]