石榴F3'H全基因组分析及其在籽粒花色苷合成中的作用  

Genome-wide analysis of the pomegranate F3’H family and their roles in aril anthocyanin biosynthesis

作　　者：陈延惠[1,2,3] 曹新悦 冯志良 谭彬 胡悦[4] 张海朋 简在海 孟海军 冯建灿[1,2,3] 万然 胡青霞[1,2,3] CHEN Yanhui;CAO Xinyue;FENG Zhiliang;TAN Bin;HU Yue;ZHANG Haipeng;JIAN Zaihai;MENG Haijun;FENG Jiancan;WAN Ran;HU Qingxia(College of Horticulture,Henan Agriculture University,Zhengzhou 450002,Henan,China;Henan Laboratory of Fruit and Cucurbit Biology,Zhengzhou 450002,Henan,China;International Joint Laboratory of Henan Horticultural Crop Biology,Zhengzhou 450002,Henan,China;Dengfeng Branch of Agricultural Bank of China,Dengfeng 452470,Henan,China)

机构地区：[1]河南农业大学园艺学院,郑州450002 [2]河南省果树瓜类生物学重点实验室,郑州450002 [3]河南省园艺植物生物学国际联合实验室,郑州450002 [4]中国农业银行登封市支行,河南登封452470

出　　处：《果树学报》2024年第6期1064-1077,共14页Journal of Fruit Science

基　　金：兵团重点领域科技攻关计划项目(2021AB015);河南省科技攻关项目(222102110479)。

摘　　要：【目的】对石榴PgF3’H进行全基因组鉴定,了解其成员大小、位置、结构、系统发育关系及顺式作用元件等相关信息,分析PgF3’H在石榴籽粒花色苷合成中的作用。【方法】通过生物信息学分析结合转录组数据了解石榴PgF3’H基因家族成员,分析其在不同品种石榴籽粒中随发育期的表达情况,并通过农杆菌介导拟南芥异源转化试验验证PgF3’H3的功能。【结果】鉴定到的3个PgF3’H成员均含有保守结构域CYP75B,具有比较保守的基因结构;对它们的顺式作用元件和表达情况进行分析,表明PgF3’H2和PgF3’H可能受光和激素调节,由MYB转录调控其表达,在石榴籽粒花色苷合成中发挥重要作用;初步验证了PgF3’H3的功能。【结论】鉴定了石榴3个PgF3’H基因成员,明确了PgF3’H2和PgF3’H3是与籽粒着色有关的重要候选基因,验证了PgF3’H3调控花色苷合成的功能。【Objective】Anthocyanins are important substance basis of coloration and intrinsic nutritional quality in Pomegranate(Punica granatum L.).Studies about anthocyanin associated genes in pomegranate are very important.F3’H is an important kind of key enzymes in anthocyanin biosynthesis pathways.However,the role of PgF3’H in aril anthocyanin biosynthesis is little known.This study introduced the PgF3’H gene family information in the whole pomegranate genome,for example,length,location,gene structure,cis-elements in promoters,and analysis of their potential roles in anthocyanin biosynthesis and coloration of pomegranate arils.【Methods】The genome of the Tunisian soft seed pomegranate(PRJNA355913)download from NCBI was used to identify the PgF3’H gene family.Their chromosomal distributions,sequences and structures,and phylogenetic relationships as well as cis-elements in promoters were analyzed through bioinformatics.Furthermore,the transcriptional expression of the PgF3’Hs based on the transcriptomic data from the arils of three pomegranate cultivars(Tunisian soft seed with red color arils,Meilisuan with dark red color and Zimei with purple red color arils)at different developental stages were analyzed to explore the possible roles of the PgF3’Hs in aril anthocyanin biosynthesis and coloration.The Agrobacterium mediated-heterogenous transformation in Arabidopsis was used to determine the PgF3’H3 function.【Results】Three PgF3’H genes in pomegranate were identified,which were renamed PgF3’H1,PgF3’H2 and PgF3’H3.Their sequences were 2055-3933 bp,their proteins contain 208-280 amino acids.The 2D and 3D protein structures were predicted,primarily withαhelices and random coils.The three PgF3’Hs together with 44 F3’H genes from other plant species were used to construct the phylogenetic tree.The results showed that the PgF3’H3/AtF3’H were orthologs and clustered in GroupⅡ,while the PgF3’H1/PgF3’H2 were paralogues and clustered in GroupⅢ.Moreover,the PgF3’H1/PgF3’H2 had a co

关 键 词：石榴 F3’H 籽粒 花色苷 颜色 

分 类 号：S665.4[农业科学—果树学]