基于代谢组学比较分析5个栽培种梨叶片代谢物的差异  

Comparative analysis of metabolites in leaves of five pear cultivars based on metabonomics

作　　者：焦慧君 冉昆[1] 董冉[1] 董肖昌 关秋竹[1] 王宏伟[1] 魏树伟[1] JIAO Huijun;RAN Kun;DONG Ran;DONG Xiaochang;GUAN Qiuzhu;WANG Hongwei;WEI Shuwei(Shandong Institute of Pomology,Tai’an 271000,Shandong,China)

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《果树学报》2024年第6期1111-1124,共14页Journal of Fruit Science

基　　金：山东省农科院创新工程项目(CXGC2023F21、CXGC2023A13);山东省果树研究所青年基金(GSS2022QN11);国家梨产业技术体系(CARS-28-37);山东省自然科学基金(ZR2020MC141、ZR2021MC177、ZR2023MC061);农业农村部园艺作物种质资源利用重点实验室开放基金(NYZS202206)。

摘　　要：【目的】为加强对梨叶片资源的药用价值以及农副产品的开发利用,全面分析梨叶片中次生代谢物质的种类。【方法】通过代谢组学分析库尔勒香梨、超红、南红、鸭梨、翠冠5个品种与福鼎大白茶的成熟期叶片的代谢物差异。【结果】PCA分析表明,代谢物种内聚类较为集中,种间聚类较远;5个比较组特异性差异代谢物分别有55、155、54、59和28种;Heatmap分析表明,排名前50的差异代谢物在5个主栽梨叶片中的表达量具有显著差异;黄酮和黄酮醇生物合成、ABC转运和类黄酮生物合成等途径是差异代谢物富集的主要通路,其中山梨醇、果糖、甘露醇、柠檬酸、水杨酸、马来酸等含量显著上调。【结论】不同栽培种梨叶片的代谢物在种类和表达量上均具有显著差异,研究结果为开发利用梨叶片资源提供了理论基础。【Objective】Plant leaves are rich in secondary metabolites,mainly including phenols,terpenoids,alkaloids and other secondary substances,which have physiological functions in regulating plant growth,biological defense,anti-abiotic stress and so on.Pear is one of the important economic fruits of Rosaceae,and widespread in the world.China is one of the origins of pears,and has a long history of cultivation and rich variety resources.Pyrus pyrifolia,P.ussuriensis,P.sinkiangensis,P.communis and P.bretschnrideri are the main cultivated species of pear in China.Pears contain a wide variety of phenolic compounds,which would be beneficial for human health.Therefore,there have been many researches on the metabolite content of pear fruit.There are also abundant secondary metabolites in pear leaves,which are very important for the growth and development of fruit trees.However,there is a lack of systematic analysis of the metabolites in pear leaves.【Methods】In this study,we collected mature,healthy pear leaves in September,and carried them into lab.The leaves of Fuding Dabai tea rich in secondary metabolites were selected as control,and the differences of metabolites in the mature leaves of five cultivars Kuerle,Chaohong,Nanhong,Yali and Cuiguan were compared and analyzed by metabolomics.PCA analysis,Heatmap analysis,KEGG analysis and K-means analysis were used to analysis differential metabolites.【Results】A total of 9011 metabolites were detected under positive and negative ion mode.11747 peaks and 4987 metabolites were identified in the positive ion model,and 11575 peaks and 4024 metabolites were identified in the negative ion model.The principal component analysis(PCA)showed that the detected intra-species clustering of metabolic species was relatively concentrated,while the interspecific clustering was relatively distant.The metabolites of the leaves of Chaohong were clustered far apart from those of the other four kinds of pear,which indicated the difference of metabolites in the leaves of occidental pear and

关 键 词：梨 叶片 次生代谢物质 

分 类 号：S661.2[农业科学—果树学]