桃基因 PpNAC 的鉴定及其在不同发育时期的表达分析  

Identification and expression analysis of PpNAC genes during different development stages of peach fruit

作　　者：王晓菲 高利盈 刘宁 程钧 王伟 谭彬[1] 郑先波[1] 叶霞[1] 冯建灿[1] 张郎郎 WANG Xiaofei;GAO Liying;LIU Ning;CHENG Jun;WANG Wei;TAN Bin;ZHENG Xianbo;YE Xia;FENG Jiancan;ZHANG Langlang(College of Horticulture,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学园艺学院,河南郑州450046

出　　处：《河南农业大学学报》2024年第3期412-423,共12页Journal of Henan Agricultural University

基　　金：国家自然科学基金青年项目(32002014);国家重点研发计划项目(2019YFD1000104)。

摘　　要：【目的】鉴定影响桃果实成熟的PpNAC基因,并分析其在桃果实不同发育时期的表达量变化,为解析桃果实成熟的分子机制奠定基础。【方法】通过对桃不同组织部位的转录组分析,从115个PpNAC基因家族成员中筛选在果实中特异表达的PpNAC基因,进一步进行生物信息学分析、蛋白互作分析和表达分析,筛选可能影响桃果实成熟的PpNAC基因。【结果】筛选到16个在桃果实中高表达的PpNAC基因,其在桃的8条染色体上都有分布,蛋白相对分子质量和等电点差异较大。基因编码区均含有NAM保守结构域和motif1~6,含有3~8个外显子,2~7个内含子,启动子中脱落酸响应元件和茉莉酸响应元件相对较多。此外,通过与已报道成熟相关NAC蛋白PpNAC1的互作筛选,得到4个与PpNAC1互作的PpNAC蛋白。进一步的表达分析表明,这4个PpNAC基因在果实成熟起始期和成熟后期表达量达到最高。【结论】PpNAC基因可能参与调控桃果实成熟。【Objective】The study aims to identify the PpNAC genes affecting peach fruit ripening and analyze its expression changes in different developmental stages of peach fruit for laying a foundation to understand the molecular mechanism of peach fruit ripening.【Method】Transcriptome analysis of different tissues were used to identify the PpNAC genes highly expressed in peach fruit from 115 PpNAC gene family members.Further bioinformatics analysis,protein interaction analysis and expression analysis were analyzed.【Result】A number of 16 PpNAC genes highly expressed in peach fruit were identified,and distributed on different 8 chromosomes.The protein molecular weight and isoelectric point were different.The coding region of gene contained the NAM conserved domain and motif1-6,including 3-8 exons and 2-7 introns.The most numerous cis-regulatory elements in promoters were involved in abscisic acid and jasmonic acid.In addition,four PpNAC proteins interacting with the PpNAC1 were obtained by interaction screening with a reported NAC member regulating peach fruit ripening.Further expression analysis showed that the expression levels of these four PpNAC genes reached the highest at the beginning and late stages of fruit ripening.【Conclusion】The PpNAC genes may participate in the regulation of peach fruit ripening.

关 键 词：桃 NAC基因 果实成熟 表达分析 

分 类 号：S662.1[农业科学—果树学]