甜瓜CmGLK转录因子调控果实品质的遗传网络解析  

Genetic network analysis of melon CmGLK transcription factor in regulating fruit quality

作　　者：郭姚淼 段世享 刘港运 李鹏飞 康旗帅 杨森 豆峻岭 胡建斌[1] 杨路明[1] 朱华玉 GUO Yaomiao;DUAN Shixiang;LIU Gangyun;LI Pengfei;KANG Qishuai;YANG Sen;DOU Junling;HU Jianbin;YANG Luming;ZHU Huayu(College of Horticulture,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学园艺学院,河南郑州450046

出　　处：《河南农业大学学报》2024年第3期424-434,共11页Journal of Henan Agricultural University

基　　金：国家自然科学基金面上项目(31872133);河南省自然科学基金优秀青年项目(212300410048);河南省高等学校青年骨干教师培养计划项目(2020GGJS045)。

摘　　要：【目的】解析甜瓜CmGLK转录因子调控果实品质的调控网络,为甜瓜果实品质改良提供理论基础。【方法】以甜瓜HB42(WT)及其Cmglk基因近等基因系glk-NIL(NIL)为试材,对其自交授粉后30 d成熟果实的果肉进行转录组学和代谢组学分析。【结果】与甜瓜果实品质相关的草酰乙酸、磷酸二羟基丙酮、3-磷酸-D-甘油酸、熊果苷、D-葡萄糖-1,6-二磷酸、9,10-环氧十八烯酸、12,13-环氧-9-十八碳烯酸等代谢物均在NIL中下调表达。转录组学数据表明,参与三羧酸循环途径的苹果酸脱氢酶基因(MDH)、E2亚基氧化戊二酸脱氢酶2基因(E 2-OGDHzhe2)在NIL中下调表达;参与糖酵解途径中的多数关键基因在NIL中下调表达,仅有丙酮酸脱氢酶基因(PDHA)、叶绿体醛酮还原酶基因(CHLAKR)、醛酮还原酶基因(AKR 4C9)、丙酮酸激酶基因(PK)4个基因在NIL中上调表达;与可溶性糖相关的β-葡萄糖苷酶15基因(BGLU 15)、β-葡萄糖苷酶12基因(BGLU 12)、β-葡萄糖苷酶45基因(BGLU 45)、β-葡萄糖苷酶42基因(BGLU 42)关键基因在NIL中下调表达;参与亚油酸代谢途径中的9个脂氧合酶基因(LOX),其中5个在NIL中是上调表达的,4个在NIL中都是下调表达的。【结论】GLK转录因子可能通过调控可溶性糖和有机酸的代谢,从而影响甜瓜果实品质,初步解析了甜瓜CmGLK转录因子调控果实品质形成的遗传调控网络。【Objective】In order to elucidate the regulatory network of melon CmGLK transcription factor regulating fruit quality and provide a theoretical basis for the improvement of melon fruit quality.【Method】The melon inbred line HB42(used as wild type)and its Cmglk near isogenic line glk-NIL(NIL)were used to conduct transcriptomic and metabolomic analysis on the pulp of mature fruits at 30 days after pollination.【Result】These metabolites related to melon fruit quality,such as oxaloacetic acid,dihydroxyacetone phosphate,3-phosphate-D-glyceroic acid,arbutin,D-glucose-1,6-diphosphate,9,10-epoxidodecenoic acid,12,13-epoxy-9-octadecenoic acid,were all down-regulated in the NIL.Transcriptomic analysis showed that the malate dehydrogenase gene(MDH)and the E2 subunit oxy glutarate dehydrogenase 2 gene(E 2-OGDHzhe2)involved in the tricarboxylic acid cycle pathway were down-regulated in the NIL.Most important genes involved in the glycolytic pathway are down-regulated in the NIL,with only four genes up-regulated in the NIL,including the pyruvate dehydrogenase gene(PDHA)and chloroplast aldosterone reductase gene(CHLAKR).The key genes related to soluble sugars includingβ-glucosidase 15 gene(BGLU 15),β-glucosidase 12 gene(BGLU 12)are down-regulated in the NIL.Five of the nine lipoxygenase genes(LOX)involved in the linoleic acid metabolism pathway are up-regulated in the NIL and four of them are down-regulated in the NIL.【Conclusion】The CmGLK transcription factors may affect melon quality by regulating the metabolism of soluble sugars and organic acids,and the genetic regulatory network of melon CmGLK transcription factor regulating fruit quality was preliminarily elucidated.

关 键 词：甜瓜 转录组学 代谢组学 GLK转录因子 果实品质 

分 类 号：S652[农业科学—果树学]