内侧前额叶皮质谷氨酸能神经元参与CD1小鼠攻击行为的机制研究  

Mechanism of glutaminergic neurons in medial prefrontal cortex involved in aggressive behavior of CD1 mice

作　　者：李加欣 黄露 龙宗泓 张敏 温惠中[2] 熊鹰[2] 李洪 白福海 LI Jiaxin;HUANG Lu;LONG Zonghong;ZHANG Min;WEN Huizhong;XIONG Ying;LI Hong;BAI Fuhai(Department of Anesthesiology,Second Affiliated Hospital,Army Medical University(Third Military Medical University),Chongqing,400037;Department of Neurobiology,College of Basic Medical Sciences,Army Medical University(Third Military Medical University),Chongqing,400038,China)

机构地区：[1]陆军军医大学(第三军医大学)第二附属医院麻醉科,重庆400038 [2]陆军军医大学(第三军医大学)基础医学院神经生物教研室,重庆400038

出　　处：《陆军军医大学学报》2024年第12期1336-1343,共8页Journal of Army Medical University

基　　金：国家自然科学基金(82101273,82171265);中国博士后面上项目(2021M693936);重庆市博士后科学基金项目(cstc2021jcyj-bshX0071);陆军军医大学科技创新能力提升专项(2021XQN19);陆军军医大学第二附属医院青年博士人才孵化计划(2023YQB007);重庆市博士后研究项目特别资助(2022CQBSHTB3021)。

摘　　要：目的研究CD1小鼠发动攻击行为的内在神经机制。方法通过驻地入侵实验筛选出具有攻击行为的CD1小鼠,攻击配对偏好进一步验证后使用c-Fos标记全脑激活的脑区,通过免疫荧光双标实验分析攻击行为激活了哪种类型的神经元,最后使用光遗传学调控该类神经元的活性,观察其对攻击行为的影响。结果通过c-Fos筛选,约82%的CD1小鼠表现出攻击行为;攻击行为发生后主要激活内侧前额叶皮质(medial prefrontal cortex,mPFC),免疫荧光双标结果显示mPFC脑区c-Fos阳性神经元主要是谷氨酸能神经元;最后,我们通过光遗传激活mPFC脑区谷氨酸能神经元,发现其能够抑制CD1小鼠的攻击行为;相反,光遗传抑制mPFC脑区谷氨酸能神经元,能够促进CD1小鼠的攻击行为。结论mPFC脑区谷氨酸能神经元是调控CD1小鼠发动攻击行为的重要组成部分。Objective To investigate the intrinsic neural mechanism of aggressive behavior in CD1 mice.Methods CD1 mice with aggressive behavior were screened out by resident intruder test.After the aggressive conditioned pair preference was further verified,the activated brain regions of the whole brain were labeled with c-Fos,and the types of neurons activated by the aggressive behavior were analyzed by double immunofluorescence labeling.Finally,the effects of activity of these neurons regulated by optogenetics on aggressive behavior were observed.Results The c-Fos screening revealed that about 82%of the CD1 mice showed aggressive behavior.After the occurrence of aggressive behavior,the main activation occured in the medial prefrontal cortex(mPFC),and the results of immunofluorescence double labeling showed that the c-Fos positive neurons in the mPFC were mainly glutamatergic neurons.Finally,glutamatergic neurons in the mPFC could be activated by optogenetics,and the activation inhibited the aggressive behavior of CD1 mice.In contrast,optogenetics could inhibit glutamatergic neurons in the mPFC and then promote the aggressive behavior of CD1 mice.Conclusion Glutamatergic neurons in the mPFC are an important component in the regulation of aggressive behavior in CD1 mice.

关 键 词：MPFC 谷氨酸能神经元 攻击行为 

分 类 号：R338.12[医药卫生—人体生理学] R338.6[医药卫生—基础医学] R394.1