慢性乙型肝炎及肝硬化患者血清血管生成素1、2及其比值变化与HBV DNA和ALT的相关性分析  

Correlation of serum angiopoietin-1,angiopoietin-2,and angiopoietin-1/angiopoietin-2 ratio with HBV DNA and alanine aminotransferase in patients with chronic hepatitis B or liver cirrhosis

作　　者：林明华[1] 常远 刘芳[1] 黄雁翔[1] 徐航飞 LIN Minghua;CHANG Yuan;LIU Fang;HUANG Yanxiang;XU Hangfei(Beijing YouAn Hospital,Beijing Institute of Liver Diseases,Capital Medical University,Beijing 100069,China;Department of Clinical Laboratory,Liaocheng People’s Hospital,Liaocheng,Shandong 252000,China)

机构地区：[1]首都医科大学附属北京佑安医院北京肝病研究所,北京100069 [2]聊城市人民医院检验科,山东聊城252000

出　　处：《临床肝胆病杂志》2024年第6期1126-1129,共4页Journal of Clinical Hepatology

基　　金：北京市属医学科研院所公益发展改革试点项目(JING YI YAN 2021-10);北京市工程技术研究中心(BG0320)。

摘　　要：目的分析血清血管生成素(Ang)1、2及其比值变化与慢性乙型肝炎(CHB)、肝硬化患者HBV DNA和ALT的相关性。方法选取2018年3月—2019年10月首都医科大学附属北京佑安医院收治的CHB患者99例,肝硬化患者59例,收集临床资料和血清标本;另选同期46例健康体检者作为对照组。所有患者均采用PCR法检测血清HBV DNA;采用酶联免疫吸附法检测血清Ang-1和Ang-2水平,比较各组血清Ang-1和Ang-2及Ang-1/Ang-2的差异。非正态分布的计量资料多组间比较采用Kruskal-Wallis H检验,进一步两两比较采用Bonferroni法;采用Spearman方法分析Ang-1、Ang-2、Ang-1/Ang-2与HBV DNA、ALT的相关性。结果与对照组(671.0 pg/mL)相比,CHB组(479.0 pg/mL)和肝硬化组(208.4 pg/mL)Ang-1水平显著降低(P值均<0.05);与CHB组相比,肝硬化组Ang-1水平显著降低(P<0.001)。与对照组(198.0 pg/mL)相比,CHB组(286.1 pg/mL)和肝硬化组(438.4 pg/mL)Ang-2水平显著升高(P值均<0.001);与CHB组相比,肝硬化组Ang-2水平显著升高(P<0.001)。与对照组(3.4)相比,CHB组(1.6)和肝硬化组(0.5)Ang-1/Ang-2比值显著降低(P值均<0.001);与CHB组相比,肝硬化组Ang-1/Ang-2比值显著降低(P<0.001)。Spearman分析显示,CHB组Ang-1与HBV DNA及ALT均呈负相关(r值分别为−0.400、−0.394,P值均˂0.001);Ang-2与HBV DNA及ALT均呈正相关(r值分别为0.365、0.351,P值均˂0.001);Ang-1/Ang-2与HBV DNA及ALT均呈负相关(r值分别为−0.463、−0.473,P值均˂0.001);而肝硬化组Ang-1、Ang-2及Ang-1/Ang-2均与HBV DNA和ALT无相关性(P值均˃0.05)。结论CHB、肝硬化患者血清Ang-1、Ang-2及Ang-1/Ang-2有显著改变,其中肝炎组Ang-1、Ang-2及Ang-1/Ang-2在一定程度上反映CHB患者肝损伤的程度。Objective To investigate the correlation of serum angiopoietin-1(Ang-1),angiopoietin-2(Ang-2),and Ang-1/Ang-2 ratio with HBA DNA and alanine aminotransferase(ALT)in patients with chronic hepatitis B(CHB)or liver cirrhosis.Methods Clinical data and serum specimens were collected from 99 patients with CHB and 59 patients with liver cirrhosis who were admitted to Beijing YouAn Hospital,Capital Medical University,from March 2018 to October 2019,and 46 individuals who underwent physical examination were enrolled as control group.PCR was used to measure serum HBV DNA level,and ELISA was used to measure the serum levels of Ang-1 and Ang-2.The serum levels of Ang-1 and Ang-2 and Ang-1/Ang-2 ratio were compared between groups.The Kruskal-Wallis H test was used for comparison of non-normally distributed continuous data between multiple groups,and the Bonferroni method was used for further comparison between two groups;the Spearman correlation analysis was used to investigate the correlation of Ang-1,Ang-2,and Ang-1/Ang-2 ratio with HBV DNA and ALT.Results Compared with the control group,the CHB group and the liver cirrhosis group had a significant reduction in the level of Ang-1(479.0 pg/mL and 208.4 pg/mL vs 671.0 pg/mL,both P<0.05),and compared with the CHB group,the liver cirrhosis group had a significant reduction in the level of Ang-1(P<0.001).Compared with the control group,the CHB group and the liver cirrhosis group had a significant increase in the level of Ang-2(286.1 pg/mL and 438.4 pg/mL vs 198.0 pg/mL,both P<0.001),and compared with the CHB group,the liver cirrhosis group had a significant increase in the level of Ang-2(P<0.001).Compared with the control group,the CHB group and the liver cirrhosis group had a significant reduction in Ang-1/Ang-2 ratio(1.6 and 0.5 vs 3.4,both P<0.001),and compared with the CHB group,the liver cirrhosis group had a significant reduction in Ang-1/Ang-2 ratio(P<0.001).The Spearman correlation analysis showed that in the CHB group,Ang-1 was negatively correlated with HBV DNA and ALT

关 键 词：血管生成素1 血管生成素2 乙型肝炎 慢性 肝硬化 乙型肝炎病毒 丙氨酸转氨酶 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]