秦川牛mtDNA全基因组遗传多样性与母系起源研究  

Genetic Diversity of Mitochondrial DNA Genome and Maternal Origins in Qinchuan Cattle

作　　者：白萌萌 马卫东 成海建[3] 黄永震[1] 雷初朝[1] BAI Meng-meng;MA Wei-dong;CHENG Hai-jian;HUANG Yong-zhen;LEI Chu-zhao(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100;Shaanxi Provincial Livestock Breeding Farm,Fufeng,Shaanxi 722203;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]陕西省农牧良种场,陕西扶风722203 [3]山东省农业科学院畜牧兽医研究所,山东济南250100

出　　处：《中国牛业科学》2024年第2期11-14,共4页China Cattle Science

基　　金：农业农村部国家肉牛牦牛产业技术体系(CARS-37)。

摘　　要：[目的]从32头秦川牛全基因组中提取其mtDNA全基因组序列进行分析,以揭示秦川牛mtDNA基因组的遗传多样性与母系起源。[方法]采用mtDNA全基因组序列比对及生物信息学方法。[结果]在32头秦川牛mtDNA全基因组序列中,共检测到30种不同的单倍型,其平均单倍型多样度(Hd±SD)为0.996±0.009,其平均核苷酸多样度(π±SD)为0.0067±0.0010,表明秦川牛具有丰富的母系遗传多样性。构建的mtDNA全基因组系统发育树与单倍型网络图表明,32头秦川牛的mtDNA全基因组序列包括T2、T3、T4、I1共4个不同的支系,其中T2支系占21.88%,T3支系占40.625%,T4支系占9.375%,I1支系占28.125%。说明秦川牛具有普通牛和瘤牛两个支系,其中普通牛支系占71.88%,瘤牛支系占28.12%。[结论]秦川牛具有丰富的母系遗传多样性,有普通牛和瘤牛两个母系起源,但以普通牛起源为主。[Objective]This study aimed to investigate the maternal origin and mitochondrial genome genetic diversity of Qinchuan cattle through the analysis of whole mitochondrial genome sequences from 32 Qinchuan cattle samples.[Methods]The study was conducted using mtDNA whole genome alignment and bioinformatics methods.[Results]Analysis revealed a total of 30 different haplotypes within the 32 mtDNA genome sequences of Qinchuan cattle.The average haplotype diversity(Hd±SD)was 0.996±0.009,and the average nucleotide diversity(π±SD)was 0.0067±0.0010,indicating significant maternal genetic diversity in Qinchuan cattle.Phylogenetic tree and haplotype network diagram construction identified four lineages(T2,T3,T4,and I1)among the mtDNA genome sequences,with lineage proportions of 21.88%,40.625%,9.375%,and 28.125%,respectively.Qinchuan cattle exhibited two main maternal origins,with Bos taurus representing 71.88%and Bos indicus at 28.12%.[Conclusion]This study concludes that Qinchuan cattle possess rich maternal genetic diversity,with two maternal origins of zebu and taurine cattle,albeit taurine cattle being the predominant origin.

关 键 词：秦川牛 mtDNA全基因组 遗传多样性 母系起源 

分 类 号：S823[农业科学—畜牧学]