大鼠急性血瘀证模型造模方法的网状Meta分析  

Network Meta-Analysis of Modeling Methods for Rats with Acute Blood Stasis Syndrome

作　　者：宋慧平[1,2] 岳傲春 李泽坤 韩伟 李琴 SONG Huiping;YUE Aochun;LI Zekun;HAN Wei;LI Qin(First School of Clinical Medicine,Shandong University of Traditional Chinese Medicine,Ji′nan 250199,China;Department of Emergency Medicine,Shenzhen University General Hospital,Shenzhen 518071,China;Medical College of Qingdao University,Qingdao 266075,China;Department of Integrated Chinese and Western Medicine,Yantai Yuhuangding Hospital Affiliated to Qingdao University,Yantai 264099,China)

机构地区：[1]山东中医药大学第一临床学院,济南250199 [2]深圳大学总医院急诊科,深圳518071 [3]青岛大学医学部,青岛266075 [4]青岛大学附属烟台毓璜顶医院中西医结合科,烟台264099

出　　处：《世界中医药》2024年第8期1099-1107,共9页World Chinese Medicine

基　　金：国家自然科学基金项目(82071465,81571283);山东省自然科学基金项目(ZR2020MH154,ZR2016HL21);山东省重点研发项目(2018GSF118215);深圳市医疗卫生三名工程项目(SZSM201911007)。

摘　　要：目的:采用网状Meta分析评价不同急性血瘀证模型造模方法的效果,为中医实验研究急性血瘀证动物模型的制备提供循证依据。方法:检索中国期刊全文数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)中有关急性血瘀证模型的建模方法,应用Stata软件进行网状Meta分析。结果:最终纳入32项研究,共纳入9种造模方案。各项指标最优前3位概率排序分别为:1)全血黏度:1 S^(-)1切变率右旋糖酐>高脂>氢化可的松+肾上腺素,10 S^(-)1切变率冰水浴>肾上腺素>高脂+力竭游泳,30 S^(-)1切变率多因素刺激>右旋糖酐>肾上腺素,60 S^(-)1切变率多因素刺激>右旋糖酐>肾上腺素,150 S^(-)1切变率肾上腺素>冰水浴>高脂+力竭游泳,200 S^(-)1切变率右旋糖酐>氢化可的松+肾上腺素>角叉莱胶+内毒素;2)血浆黏度:右旋糖酐>多因素刺激>肾上腺素;3)红细胞聚集指数:冰水浴>多因素刺激>高脂+力竭游泳。受纳入研究质量和数量的限制,所得结论仍待进一步验证。结论:右旋糖酐、冰水浴、多因素刺激、肾上腺素造模效果更显著。Objective:To evaluate the effect of different modeling methods for acute blood stasis syndrome by network meta-analysis and provide an evidence-based basis for the preparation of an animal model with acute blood stasis syndrome in research of traditional Chinese medicine(TCM).Methods:The modeling methods for acute blood stasis syndrome were searched from Databases of China National Knowledge Infrastructure(CNKI),China Science Periodical Database(CSPD),and Chinese Citation Database(CCD).The network meta-analysis was carried out by Stata software.Results:A total of 32 pieces of literature and nine modeling schemes were finally included.The best first three probability indexes were ranked as follows:1) whole blood viscosity:1 S^(-1) shear rate dextran>high fat>hydrocortisone+adrenaline,10 S^(-1) shear rate ice water bath>adrenaline>high-fat feeding+exhaustive swimming,30 S^(-1) shear rate multifactor stimulation>dextran>adrenaline,60 S^(-1) shear rate multifactor stimulation>dextran>adrenaline,150 S^(-1) shear rate adrenaline>ice water bath>high-fat feeding+exhaustive swimming,200 S^(-1) shear rate dextran>hydrocortisone+adrenaline>carrageenan+endotoxin;2) plasma viscosity:dextran>multifactor stimulation>adrenaline;3)erythrocyte aggregation index:ice water bath>multifactor stimulation>high-fat feeding+exhaustive swimming.Limited by the quality and quantity of the included studies,the conclusions still need to be further verified.Conclusion:dextran,ice water bath,multifactor stimulation,and adrenaline are more effective in modeling.

关 键 词：急性血瘀证 动物模型 造模方法 大鼠 模型评价 实验方法 网状Meta分析 系统评价 

分 类 号：R255.7[医药卫生—中医内科学]