基于谱效关系的款冬花祛痰活性成分研究  

Bioactive Components in Farfarae Flos for Dispelling Phlegm:A Study Based on Spectrum-effect Relationship

作　　者：吴笛 罗文汇[3] 赵光发 何默忠 WU Di;LUO Wenhui;ZHAO Guangfa;HE Mozhong(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Key Laboratory of Research on Prevention and Treatment of Major Diseases in Chinese Internal Medicine and Transformation of Achievements of Ministry of Education,Changsha 410007,China;Hunan Yifang Tianjiang Pharmaceutical Co.,Ltd.,Changde 415001,China)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]中医内科重大疾病防治研究及转化教育部重点实验室,长沙410007 [3]湖南一方天江药业有限公司,常德415001

出　　处：《世界中医药》2024年第9期1246-1251,共6页World Chinese Medicine

基　　金：湖南省自然科学基金科药联合基金项目(2021JJ80067)——基于入血成分谱效关系研究款冬花镇咳、祛痰药效物质基础;湖南省“国内一流培育学科”药学开放基金项目(2021YX03)——基于LPS诱导大鼠慢性支气管炎模型的紫菀散抗炎作用研究。

摘　　要：目的:研究款冬花提取物的高效液相色谱指纹图谱与祛痰作用的谱效关系,筛选款冬花的祛痰活性成分。方法:采用高效液相色谱法建立款冬花提取物的指纹图谱并获得其化学成分含量信息,采用小鼠酚红排泌模型获得款冬花提取物的祛痰药效指标信息,采用偏最小二乘回归法对款冬花提取物的化学成分含量信息与祛痰药效指标信息进行谱效相关分析。结果:初步判断芦丁、异槲皮苷、绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C及峰3成分、峰17成分构成款冬花的祛痰活性成分群。结论:款冬花的祛痰作用是多成分共同作用的结果。本研究结果可为关联药效的款冬花质量标准的建立提供参考。Objective:To study the relationship between the high performance liquid chromatography(HPLC) fingerprint and the phlegm-dispelling effect of Farfarae Flos extract,and clarify the bioactive components of Farfarae Flos for dispelling phlegm.Methods:HPLC was employed to establish the fingerprint of Farfarae Flos extract and determine the content of chemical components.The phlegm-dispelling effect of Farfarae Flos extract was evaluated based on the volume of phenol red in mouse trachea.The spectrum-effect relationship was analyzed by partial least squares regression.Results:The bioactive components in Farfarae Flos extract for dispelling phlegm included rutin,isoquercitrin,chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C,and the components corresponding to peaks 3 and 17.Conclusion:The phlegm-dispelling effect of Farfarae Flos was the result of the joint actions of multiple components.This study provides a reference for establishing an effect-related quality standard of Farfarae Flos.

关 键 词：款冬花 高效液相色谱 指纹图谱 小鼠气管酚红排泌 祛痰作用 偏最小二乘回归法 谱效关系 活性成分 

分 类 号：R284[医药卫生—中药学]