人类微量精子冷冻载体的研究进展  

Research progress on the cryopreservation carriers of small quantity of human spermatozoa

作　　者：李娅娅 陈耀平[2,3] 徐仙 LI Yaya;CHEN Yaoping;XU Xian(Ningxia Medical University,Yinchuan,Ningxia,750004;General Hospital of Ningxia Medical University,Yinchuan;Key Laboratory of Fertility Preservation and Maintenance,Ministry of Education,Ningxia Medical University,Yinchuan)

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院 [3]宁夏医科大学生育力保持教育部重点实验室

出　　处：《中国计划生育学杂志》2024年第6期1457-1463,共7页Chinese Journal of Family Planning

基　　金：宁夏回族自治区重点研发计划项目(2021BEG03114)。

摘　　要：全球不孕不育症中男性因素约占50%,其中无精子症患者通常需要通过手术获取微量精子,或极重度少弱精子症患者的精子数量稀少,采用常规冷冻载体冷冻复苏后难以寻找到存活的精子。因此为避免取卵日无精子可用而取消周期,临床上应用微量精子冷冻载体预先冷冻少量精子,如空卵透明带、海藻酸胶囊、微滴法、麦管法、薄片法、剥卵针等。本文就人类微量精子的不同冷冻载体的研究进行综述,为临床上优化微量精子的冷冻方法提供理论依据。Male factors account for about 50%of global infertility,among which,the patients with azoospermia usually need to obtain the small amount of sperm through surgery,or the patients with very severe oligoasthenospermia have a small number of sperm,so it is difficult to find viable sperm after freezing resuscitation with conventional frozen carriers.Therefore,in order to avoid the cancellation of the cycle because of the unavailable sperm on the day of egg retrieval,the small number of sperm are clinically frozen by the microsperm freezing carriers in advance,such as empty egg zona,alginate capsules,micro-drop method,straws,pieces,glass denuding pipette,etc.In this paper,the research on the different cryopreservation carriers of the small quantity of the patients with spermatozoa was reviewed,so as to provide the theoretical basis for optimizing cryopreservation methods of the microsperm of the patients in clinic.

关 键 词：男性不育 微量精子 冷冻载体 精子冷冻保存 回收率 

分 类 号：R714.8[医药卫生—妇产科学]