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作 者:彭玉龙 任延靖 赵孟良[1] PENG Yulong;REN Yanjing;ZHAO Mengliang(Qinghai University,Xining Qinghai 810016,China)
机构地区:[1]青海大学,青海西宁810016
出 处:《青海农林科技》2024年第2期1-7,共7页Science and Technology of Qinghai Agriculture and Forestry
基 金:青海省科技厅应用基础研究项目(2022-ZJ-736)。
摘 要:为研究不同逆境胁迫对菊芋幼苗叶片生理特性的影响,探究菊芋生长过程中生理特性的变化规律,以青芋1号菊芋品种块茎为试验材料,分别采用25%PEG-6000模拟干旱胁迫和200 mmol/L NaCl溶液模拟盐胁迫对菊芋幼苗进行处理,以清水处理为对照。在处理后的24 h、48 h、72 h取样测定菊芋幼苗叶片中的生理指标。结果表明:在干旱胁迫、盐胁迫处理下菊芋叶片中还原型谷甘光肽(GSH)、抗坏血酸(AsA)、可溶性糖(SS)含量均显著低于对照;可溶性蛋白(SP)含量则试验组均高于对照组;抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)活性、丙二醛(MDA)、过氧化氢(H 2 O 2)含量总体呈先上升后下降趋势,本研究的结果为后续菊芋抗逆育种提供参考。In order to study the effects of different stress on leaf physiological characteristics of Jerusalem artichoke(JA)seedlings,and to explore the changes of physiological characteristics during the growth of JA seedlings,the tubers of Qingyu No.1 JA seedlings were treated with 25%PEG-6000 simulated drought stress and 200 mmol/L NaCl solution simulated salt stress,respectively,and water treatment as the control.The physiological indexes in the leaves of JA seedlings were measured at 24 h,48 h and 72 h after treatment.The results showed that the contents of reduced glutamylglycophatide(GSH),ascorbic acid(AsA)and soluble sugar(SS)in leaves of JA under drought and salt stress were significantly lower than those of control.The content of soluble protein(SP)in experimental groups was higher than that in control group.The activities of ascorbate peroxidase(APX),catalase(CAT),malondialdehyde(MDA)and hydrogen peroxide(H 2O 2)were firstly increased and then decreased.The results of this study provide a reference for subsequent stress-resistant breeding of Jerusalem artichoke.
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