Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging  

作　　者：王柯欣 余文慧 屈军乐 廖常锐 王义平 何俊 刘丽炜 Kexin Wang;Wenhui Yu;Junle Qu;Changrui Liao;Yiping Wang;Jun He;Liwei Liu(State Key Laboratory of Radio Frequency Heterogeneous Integration(Shenzhen University),Shenzhen 518060,China;Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province&Ministry of Education,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]State Key Laboratory of Radio Frequency Heterogeneous Integration(Shenzhen University),Shenzhen 518060,China [2]Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province&Ministry of Education,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China

出　　处：《Chinese Optics Letters》2024年第4期89-94,共6页中国光学快报（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2021YFF0502900);the National Natural Science Foundation of China(Nos.62175163,62225505,61935012,61835009,62127819,and 62205220);the Shenzhen Key Projects(No.JCYJ20200109105404067);the Shenzhen Talent Innovation Project(No.RCJC20210706091949022);the Shenzhen Science and Technology Planning Project(No.ZDSYS20210623092006020)。

摘　　要：Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively.However,the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues.This paper introduces an iterative multi-photon adaptive compensation technique(IMPACT)into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues.It shows that 400 correction modes can be achieved within 5 min,which was mainly limited by the frame rate of a spatial light modulator.This system was used for high-resolution imaging of mice brain tissue and live zebrafish,further verifying its superior performance in imaging quality and photon accumulation speed.

关 键 词：adaptive optics iterative optimization two-photon fluorescence lifetime imaging microscopy wavefront correction 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]