不同来源黄连花薹的自由基清除活性及其物质基础  

Radicals Scavenging Activities and Material Bases of Coptis Inflorescences from Different Sources

作　　者：孙婉春 马冰馨 王有为[1] 周高[1] 赵光年 SUN Wanchun;MA Bingxin;WANG Youwei;ZHOU Gao;ZHAO Guangnian(School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,Hubei,China;Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China)

机构地区：[1]武汉大学药学院,湖北武汉430071 [2]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《武汉大学学报（理学版）》2024年第2期213-220,共8页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金青年科学基金(82004017);湖北省自然科学基金面上项目(2023AFB670)。

摘　　要：为了研究不同来源黄连花薹的自由基清除活性及其物质基础,对不同来源黄连花薹(湖北省利川市味连、重庆市石柱县味连、四川省洪雅县雅连和云南省福贡县云连)乙醇提取物中总酚、总花青素、总生物碱、总黄酮及蒙花苷的含量进行检测,对其清除DPPH自由基(DPPH^(•))、ABTS自由基(ABTS^(•+))和超氧阴离子自由基(O_(2)^(•-))的活性进行分析。结果表明:总酚与总黄酮含量以雅连花薹乙醇提取物中最高,分别为118.26±7.07 mg/g(以没食子酸计)和78.39±4.92 mg/g(以芦丁计);总花青素与总生物碱含量以云连花薹乙醇提取物中最高,分别为9.83±2.00 mg/g(以儿茶素计)和86.46±0.38 mg/g(以盐酸小檗碱计);利川味连与石柱味连花薹中总生物碱含量间无显著性差异(P>0.05)。不同来源黄连花薹乙醇提取物对DPPH^(•)、ABTS^(•+)和O_(2)^(•-)的清除活性由强到弱依次为:雅连花薹、石柱味连花薹、利川味连花薹及云连花薹。蒙花苷对DPPH^(•)、ABTS^(•+)和O_(2)^(•-)的清除活性均较低,分别为4.88%~5.54%、12.17%~16.82%和11.37%~12.21%。分子对接结果表明,影响蒙花苷清除自由基活性的主要原因是蒙花苷给电子能力较弱,推测这是蒙花苷对3种自由基的清除活性贡献率较低的内在原因。In order to study the radicals scavenging activities and main material bases of coptis inflorescences from different sources,the contents of total phenols,total anthocyanins,total alkaloids,total flavonoids and linarin in alcohol extracts of coptis inflorescences from different sources(coptis inflorescences from Lichuan,Hubei and Shizhu,Chongqing;Coptis deltoidea inflorescence from Hongya,Sichuan;Coptis teeta inflorescence from Fugong,Yunnan)were quantitatively detected.At the same time,the scavenging activities of DPPH radical(DPPH^(•)),ABTS radical(ABTS^(•+))and superoxide anion radical(O_(2)_(•-))in alcohol extracts of coptis inflorescences from different sources were tested and analyzed.The results showed that the total phenolic and total flavonoid contents in Coptis deltoidea inflorescence are the highest among all the coptis inflorescences,at the level of 118.26±7.07 mg/g(calculated by gallic acid)and 78.39±4.92 mg/g(calculated by rutin),respectively.The contents of total anthocyanins and total alkaloids in Coptis teeta inflorescence are relatively high,at the level of 9.83±2.00 mg/g(calculated by catechin)and 86.46±0.38 mg/g(calculated by berberine hydrochloride),respectively.The total alkaloid contents in Coptis chinensis inflorescences from Lichuan and Shizhu are no significant difference(P>0.05).Radicals scavenging activities of coptis inflorescences from different sources on DPPH_(•),ABTS^(•+)and O_(2)^(•-),from strong to weak,are Coptis deltoidea inflorescence,Coptis chinensis inflorescence from Shizhu,Coptis chinensis inflorescence from Lichuan,Coptis teeta inflorescence.The radicals scavenging activities of linarin on DPPH^(•),ABTS^(•+)and O_(2)^(•-)are 4.88%to 5.54%,12.17%to 16.82%,and 11.37%to 12.21%,respectively.The molecular docking results indicate that the main reason affecting radicals scavenging activity of linarin is its weak electron donating ability,which is speculated to be the intrinsic reason for the low contribution rate of linarin to radicals scavenging activiti

关 键 词：黄连花薹 药食同源 蒙花苷 自由基清除活性 物质基础 

分 类 号：R93[医药卫生—生药学]