黄连花薹及其生物碱的急性与亚急性毒性研究  

Acute and Sub-Acute Oral Toxicities of Coptis chinensis Inflorescence and Its Alkaloids Mixture

作　　者：马冰馨 莫启贵[1] 王有为[1] 童静[1] 周高[1] 何敬胜[1] 赵光年 MA Bingxin;MO Qigui;WANG Youwei;TONG Jing;ZHOU Gao;HE Jingsheng;ZHAO Guangnian(School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,Hubei,China;Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China)

机构地区：[1]武汉大学药学院,湖北武汉430071 [2]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《武汉大学学报（理学版）》2024年第2期253-260,共8页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金青年科学基金(82004017);湖北省自然科学基金面上项目(2023AFB670)。

摘　　要：为调查黄连花薹作为可食用资源的安全性,开展了黄连花薹及其主要生物碱的急性毒性和亚急性毒性试验。在急性毒性试验中,用20000 mg/kg黄连花薹提取物和743.2 mg/kg生物碱混合物分别灌胃KM小鼠,14 d内未发现任何毒性反应且无动物死亡现象。在亚急性毒性试验中,以500、1000、2000 mg/kg 3个剂量的黄连花薹提取物及37.16 mg/kg的生物碱混合物分别灌胃SD大鼠28 d,对照组和各处理组的动物体重和器官相对质量没有显著性差异,生化、血液和尿液指标检查均在正常范围内,主要脏器无病变,而且未观察到动物死亡现象。上述实验结果证明了黄连花薹作为药食同源原料的安全性。In order to study the safety of Coptis chinensis inflorescence as an edible raw material,the acute and sub-acute oral toxicities of C.chinensis inflorescence extract(CE)and its main alkaloids(MIX)were tested.KM mice were orally administered with 20000 mg/kg CE or 743.2 mg/kg MIX for 14 days,and no toxicity or mortality was observed.SD rats were orally administered with three different doses of CE(500,1000,2000 mg/kg)and the alkaloids mixture(37.16 mg/kg)for 28 days.There was no significant difference detected in animal weight and organ relative weight between the control group and the treatment groups.The serum biochemical,hematological and urine parameters of all groups were examined and found to be within the normal ranges.Finally,the main organs did not present any histopathological lesions,and no animal death was observed.Based on these results,the safety of C.chinensis inflorescence as a medicinal and food homologous raw material can be ensured.

关 键 词：黄连花薹 药食同源 生物碱 急性毒性 亚急性毒性 

分 类 号：R932[医药卫生—生药学]