基于Ghrelin-UCP2通路探讨电针合谷、太冲穴改善帕金森病小鼠运动及非运动功能障碍的作用机制  

Mechanism of electroacupuncture at Hegu(L14)and Taichong(LR3)improving motor and non-motor dysfunction in Parkinson's disease mice based on via Ghrelin-UCP2 pathway

作　　者：常晓丽 张丽丽[1] 徐子铭 高靖然 张海燕[1] 李昭凤[1] 陈少宗[1] CHANG Xiaoli;ZHANG Lili;XU Ziming;GAO Jingran;ZHANG Haiyan;LI Zhaofeng;CHEN Shaozong(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,济南250355

出　　处：《中华中医药杂志》2024年第5期2406-2410,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点研发计划项目(No.2019YFC1712105);国家自然科学基金项目(No.82205290);山东省自然科学基金项目(No.ZR2023QH041);山东省中医药科技项目(No.2020Q007);山东中医药大学青年科研创新团队项目(No.22202110)。

摘　　要：目的:探讨电针合谷、太冲穴改善帕金森病(PD)小鼠运动及非运动功能障碍的机制。方法:雄性C57BL/6小鼠随机分为对照组、模型组、电针组和假电针组。腹腔注射甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导PD模型,电针合谷、太冲穴干预14 d,1次/d,每次20 min。爬杆实验、转棒实验和步态分析实验检测小鼠运动功能,旷场实验检测焦虑样行为,多导电生理记录结肠放电活动;ELISA法检测血清胃饥饿素(Ghrelin)含量;Western Blot检测黑质酪氨酸羟化酶(TH)和解偶联蛋白2(UCP2)的表达。结果:与模型组比较,电针组小鼠在转棒仪坚持的时间显著延长(P<0.01),爬杆时间显著缩短(P<0.01)及步态障碍现在得到改善;在旷场中央区停留时间及进入中央区的次数显著增加(P<0.01);结肠放电频率显著增加(P<0.01);黑质TH、UCP2蛋白及血清Ghrelin含量显著上调(P<0.01)。结论:电针合谷、太冲可能通过调控Ghrelin,激活Ghrelin-UCP2通路发挥神经保护作用,改善PD小鼠运动及非运动功能障碍。Objective:To investigate the mechanism of electroacupuncture improving motor and non-motor functions in Parkinson's disease(PD)mice.Methods:Male C57BL/6 mice were randomly divided into control group,model group,electroacupuncture group and sham electroacupuncture group.The PD model was established by intraperitoneal injection of MPTP,and electroacupuncture at Hegu(L14)and Taichong(LR3)points for intervention for 14 days,with a duration of 20 min per session,once a day.The motor function was assessed by climbing rod,rotating rod and gait analysis experiment,anxiety like behavior was detected by open field test,and colon firing activity was recorded multi conductive physiological;the concentration of serum ghrelin was detected by ELISA;Western Blot was used to detect the expression of tyrosine hydroxylase(TH)and uncoupling protein 2(UCP2)in the substantia nigra.Results:Compared with the model group,the duration of stick rotation of the electroacupuncture group was significantly longer(P<0.01),the climbing time was significantly shorter(P<0.01)and the gait disorders improved.In stay time in the central area of the open field and the number of times entering the central area was significantly increased(P<0.01),the frequency of colonic discharge was significantly increased(P<0.01).The substantia nigra TH,UCP2 protein,and serum Ghrelin were significantly up-regulated(P<0.01).Conclusion:Electroacupuncture at L14 and LR3 can significantly improve motor and non-motor dysfunction in PD mice,which may exert neuroprotective effects by regulating Ghrelin and activating the Ghrelin-UCP2 signaling pathway.

关 键 词：帕金森病 电针 胃饥饿素 解偶联蛋白2 黑质 合谷 太冲 

分 类 号：R245.97[医药卫生—针灸推拿学]