从中药材料学角度研究莲子心抗肿瘤药效物质基础  

Research on material basis of lotus plumule for anti-tumor from medicine-hebology perspective

作　　者：杨妮[1] 曹鹏[1] 陈景 YANG Ni;CAO Peng;CHEN Jing(Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学,南京210023

出　　处：《中华中医药杂志》2024年第5期2430-2433,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82305052);中国科学技术协会青年人才托举工程项目(No.2022-QNRC1-04);江苏省特聘教授基金项目(No.013074004038)。

摘　　要：目的:以中药材料学角度研究莲子心抗肿瘤药效物质基础。方法:通过动态光散射仪(DLS)和透射电镜(TEM)对甲基莲心碱/锰离子/人血清白蛋白复合纳米粒的粒径和形态进行表征。分别采用CCK-8法和活性氧检测试剂盒评估甲基莲心碱/锰离子/人血清白蛋白复合纳米粒对4T1细胞的毒性和产生活性氧的能力。分别将脂多糖、白介素-4、甲基莲心碱、锰离子和甲基莲心碱/锰离子/人血清白蛋白复合纳米粒与RAW264.7细胞共孵育24 h,并采用流式细胞术检测M1和M2型巨噬细胞占比来评估甲基莲心碱/锰离子/人血清白蛋白复合纳米粒重编程巨噬细胞的能力。进一步构建Balb/c小鼠4T1肿瘤模型,并对甲基莲心碱/锰离子/人血清白蛋白复合纳米粒的体内抗肿瘤活性进行评估。结果:甲基莲心碱/锰离子/人血清白蛋白复合纳米粒粒径约为148 nm呈圆形均匀分布。CCK-8实验结果显示:甲基莲心碱/锰离子/人血清白蛋白复合纳米粒对4T1细胞存在浓度依赖性细胞杀伤。进一步的机制研究结果表明:与甲基莲心碱组比较,其能提高4T1细胞内活性氧水平,高效抑制M2型巨噬细胞的极化。体内抗肿瘤实验证实了甲基莲心碱/锰离子/人血清白蛋白复合纳米粒抗肿瘤疗效强于甲基莲心碱(P<0.05)。结论:甲基莲心碱/锰离子/人血清白蛋白复合纳米粒被证实是莲子心抗肿瘤新型药效物质。Objective:To study the material basis of the anti-tumor efficacy of lotus plumule from the perspective of medicine-hebology.Methods:The particle size and morphology of neferine/manganese ion/human serum albumin composite nanoparticle was characterized by dynamic light scattering(DLS)and transmission electron microscope(TEM).The toxicity and ability to generate reactive oxygen species of neferine/manganese ion/human serum albumin composite nanoparticle on 4T1cells was evaluated by CCK-8 assay and reactive oxygen species assay kit,respectively.The lipopolysaccharide,interleukin-4,neferine,manganese ion and neferine/manganese ion/human serum albumin composite nanoparticle were co-incubated with RAW264.7 cells for 24 h each and the ability of the neferine/manganese ion/human serum albumin composite nanoparticle to reprogramme macrophages was evaluated by detecting the percentage of M1 and M2 type macrophages using flow cytometry.4T1tumor-bearing Balb/c mouse model was used to further evaluate the antitumor ability of neferine/manganese ion/human serum albumin composite nanoparticles in vivo.Results:In ultrapure water,the hydrodynamic diameter of neferine/manganese ion/human serum albumin composite nanoparticle was about 148 nm,The result of TEM showed that the neferine/manganese ion/human serum albumin composite nanoparticle was rounded and uniformly distributed.The CCK-8 experiments showed that there was a concentration-dependent cell killing of 4T1 cells by neferine/manganese ion/human serum albumin composite nanoparticle.Further mechanistic findings showed that it increased intracellular reactive oxygen species levels in 4T1 cells and effectively inhibited M2-type macrophage polarisation compared to the free neferine group.In vivo anti-tumor experiments confirmed that the anti-tumor efficacy of neferine/manganese ion/human serum albumin composite nanoparticles was stronger than that of neferine(P<0.05).Conclusion:The neferine/manganese ion/human serum albumin composite nanoparticle is found to be the novel antitum

关 键 词：莲子心 甲基莲心碱 药效物质基础 抗肿瘤 中药材料学 

分 类 号：R285[医药卫生—中药学]