补肾壮筋汤多种成分的定量和定性分析  

Quantitative and qualitative analysis of various components of Bushen Zhuangjin Decoction

作　　者：许云腾 黄思贤 施颖 易周萍 茆敏 段辛威 王丽丽 李西海 XU Yunteng;HUANG Sixian;SHI Ying;YI Zhouping;MAO Min;DUAN Xinwei;WANG Lili;LI Xihai(Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Pharmacy Science,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fuzhou 350122,China;College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学中西医结合研究院,福州350122 [2]福建中医药大学药学院,福州350122 [3]福建省中西医结合老年性疾病重点实验室,福州350122 [4]福建中医药大学中西医结合学院,福州350122

出　　处：《中华中医药杂志》2024年第5期2451-2455,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82074461,No.82305267);福建省自然科学基金项目(No.2022J01371)。

摘　　要：目的:建立高效液相色谱法(HPLC)对补肾壮筋汤多种活性成分进行定性定量分析。方法:冻干粉制备:制备补肾壮筋汤水提液,减压旋转浓缩,制备冻干粉。HPLC法:分析采用Agilent EC-C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为水-乙腈,梯度洗脱;检测波长为212 nm(梓醇)、230 nm(松脂醇二葡萄糖苷)和240 nm(莫诺苷、芍药苷、橙皮苷),流速为1 mL/min,柱温为30℃。结果:梓醇、莫诺苷、松脂醇二葡萄糖苷、芍药苷和橙皮苷线性关系良好,R^(2)均>0.9800,平均加样回收率分别为87.63%、94.11%、89.49%、86.80%、110.34%。1 g补肾壮筋汤中药复方中,梓醇含量为5.0010 mg,莫诺苷含量为1.0340 mg,松脂醇二葡萄糖苷含量为0.0772 mg,芍药苷含量为0.4224 mg,橙皮苷含量为4.5980 mg。结论:该方法可行性好、灵敏度高,适用于补肾壮筋汤的质量控制。Objective:High performance liquid chromatography(HPLC)method was established for the qualitative and quantitative analysis of various active ingredients in Bushen Zhuangjin Decoction.Methods:Preparation of lyophilized powder:the water extract of the Bushen Zhuangjin Decoction was prepared and concentrated under reduced pressure to prepare lyophilized powder.HPLC analysis was performed on an Agilent EC-C_(18)(4.6 mm×250 mm,5μm)column.The mobile phase was wateracetonitrile with gradient elution.The detection wavelengths were 212 nm(catalpol),230 nm(pinoresinol diglucoside)and 240 nm(monoside,paeoniflorin,and hesperidin).The flow rate was 1 mL/min,and the column temperature was 30℃.Results:The linear relationship of catalpa,morroniside,pinoresinol diglucoside,paeoniflorin,and hesperidin was good,R^(2)≥0.9800,and the average recovery rates were 87.63%,94.11%,89.49%,86.80%,110.34%,respectively.In 1 g of Bushen Zhuangjin Decoction,the content of catalpa was 5.0010 mg,the content of monoside was 1.0340 mg,the content of pinerinol diglucoside was0.0772 mg,the content of paeoniflorin was 0.4224 mg,and the content of hesperidin was 4.5980 mg.Conclusion:The method is feasible,sensitive,and suitable for the quality control of Bushen Zhuangjin Decoction.

关 键 词：高效液相色谱法 补肾壮筋汤 化学成分 含量测定 

分 类 号：R286.0[医药卫生—中药学]