膈下逐瘀汤加减方通过lncRNAs/NLRP3炎症小体通路抑制慢性输卵管炎大鼠细胞焦亡  

Modified Gexiazhuyu Decoction inhibits pyroptosis via lncRNAs/NLRP3 inflammasome pathway in rats with chronic salpingitis

作　　者：袁孟珂 刘进哲 穆姿辰 刘丽[3] YUAN Mengke;LIU Jinzhe;MU Zichen;LIU Li(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]贵州中医药大学,贵阳550025 [3]黑龙江中医药大学附属第一医院,哈尔滨150040

出　　处：《中华中医药杂志》2024年第5期2582-2586,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省自然科学基金面上项目(No.H2015026);中央支持地方高校改革发展资金人才培养项目(No.2021ZYGLG001)。

摘　　要：目的:从抑制细胞焦亡的角度探索膈下逐瘀汤加减方治疗慢性输卵管炎(CS)的作用机制。方法:50只雌性SD大鼠随机分为空白组,模型组,膈下逐瘀汤加减方低、中、高剂量组。膈下逐瘀汤加减方低、中、高剂量组分别给予0.9、1.8、3.6 g/kg膈下逐瘀汤加减方灌胃,空白组、模型组给予等体积纯净水。HE染色观察输卵管病理,qRT-PCR检测组织lncT048561、lncENST00000602957及NLRP3、caspase-1、GSDMD、IL-18、IL-1β mRNA表达。Western Blot检测NLRP3、procaspase-1、cleaved caspase-1、GSDMD-N蛋白表达。ELISA检测血清IL-18、IL-1β表达。结果:与空白组比较,模型组输卵管炎性损伤明显,lncT048561、lncENST0000060295、NLRP3、caspase-1、GSDMD、IL-18、IL-1β mRNA增高,NLRP3、cleaved caspase-1、GSDMD-N蛋白和血清IL-18、IL-1β增高(P<0.05)。与模型组比较,除GSDMD mRNA、NLRP3蛋白在膈下逐瘀汤加减方低剂量组变化不明显外,膈下逐瘀汤加减方各剂量组以上指标均能改善(P<0.05,P<0.01)。结论:膈下逐瘀汤加减方能够抑制lncRNAs/NLRP3炎症小体通路介导的细胞焦亡治疗CS。Objective:To explore the therapeutic mechanism of modified Gexiazhuyu Decoction(GXZYD)on chronic salpingitis(CS)from the perspective of inhibiting pyroptosis.Methods:Fifty female SD rats were randomly divided into the control group,model group,GXZYD-low,-medium and-high dose groups.GXZYD-low,-medium and-high dose groups were gavaged with 0.9,1.8,3.6 g/kg of GXZYD,control group and model group were gavaged with equal volume purified water.HE staining was used to assess tubal pathology,qRT-PCR was used to detect tubal lncT048561,lncENST00000602957,and mRNAs levels of NLRP3,caspase-1,GSDMD,IL-18 and IL-1β.Western Blot was used to detect tubal NLRP3,procaspase-1,cleaved caspase-1 and GSDMD-N proteins,and ELISA was used to test serum IL-18 and IL-1βexpressions.Results:Compared with the control group,tubal inflammatory injury was obvious in the model group,and lncT048561,lncENST0000060295,and mRNAs of NLRP3,caspase-1,GSDMD,IL-18 and IL-1βwere all increased(P<0.05).The protein levels of NLRP3,cleaved caspase-1,GSDMD-N,and serum IL-18 and IL-1βwere all increased(P<0.05).Compared with the model group,GXZYD each dose groups improved these indexes(P<0.05,P<0.01),with the exception of GSDMD mRNA and NLRP3 protein remained unchanged in the GXZYD-low dose group.Conclusion:GXZYD inhibits lncRNAs/NLRP3 inflammasome mediated pyroptosis and alleviate CS.

关 键 词：慢性输卵管炎 气滞血瘀 细胞焦亡 膈下逐瘀汤加减方 NOD样受体热蛋白结构域相关蛋白3 长链非编码RNA 

分 类 号：R285.5[医药卫生—中药学]