基于血清代谢组学探究益艾康胶囊治疗艾滋病免疫重建不良患者的作用机制  

Exploration on the mechanism of Yiaikang Capsules in the treatment of AIDS immune deficiency syndrome based on metabolomics

作　　者：陈莉华[1,2] 桑锋 许前磊[1,3] 李鹏宇 王丹妮[1,3] 郭会军 张辉[1] 吴智慧 CHEN Lihua;SANG Feng;XU Qianlei;LI Pengyu;WANG Danni;GUO Huijun;ZHANG Hui;WU Zhihui(The First Affiliated Hospital of Henan University of CM,Zhengzhou 450004,China;The First Clinical Medical School,Henan University of Chinese Medicine,Zhengzhou 450000,China;Henan Key Laboratory of Viral Diseases Prevention and Treatment of Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]河南中医药大学第一附属医院,郑州450000 [2]河南中医药大学第一临床医学院,郑州450000 [3]河南省中医药防治病毒性疾病重点实验室,郑州450000

出　　处：《中华中医药杂志》2024年第5期2651-2656,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82274474);河南省科技攻关课题(No.202102310165,No.222102310570,No.232102311207);河南省第二批中医药青苗人才培养项目(No.豫卫中医函[2021]16号);河南省中医学“双一流”创建科学研究专项(No.HSRPDFCTCM-2023-3-22)。

摘　　要：目的:运用血清代谢组学的方法探究益艾康胶囊治疗艾滋病免疫重建不良的作用机制。方法:纳入83例艾滋病免疫重建不良患者,其中肺脾气虚证患者58例,非肺脾气虚证患者25例。给予肺脾气虚证患者益艾康胶囊干预24周,用流式细胞技术检测各样本T淋巴细胞亚群及CD_(4)^(+)/CD_(8)^(+)细胞比例;利用质谱技术检测干预前后各组血清,对代谢组学数据分析整理。结果:益艾康胶囊干预后CD_(4)^(+)T细胞计数、CD_(4)^(+)/CD_(8)^(+)比值均显著升高(P<0.05)。肺脾气虚组和非肺脾气虚证组鉴别出代谢差异物296种,肺脾气虚证治疗前后鉴别出差异代谢物248种。将差异代谢产物进行代谢通路的富集分析,肺脾气虚证组和非肺脾气虚组、益艾康胶囊治疗前后共同差异代谢通路为硒氨基酸代谢、泛酸和CoA生物合成、磷酸肌醇代谢、类固醇激素的合成、烟酸酯和烟酰胺代谢、嘌呤代谢。共同的代谢通路命中的代谢物1-磷脂酰-D-巯基肌醇、PC[18:3(9Z,12Z,15Z)/20:1(11Z)]、PE(14:0/P-18:0)、喹啉酸、去氧皮质酮、硒代半胱氨酸。结论:益艾康胶囊可以促进免疫功能重建,1-磷脂酰-D-巯基肌醇、PC[18:3(9Z,12Z,15Z)/20:1(11Z)]、PE(14:0/P-18:0)、喹啉酸、去氧皮质酮、硒代半胱氨酸等代谢产物与益艾康胶囊治疗肺脾气虚证作用紧密相关,其所在代谢通路可能是益艾康胶囊治疗免疫重建不良肺脾气虚证潜在切入点。Objective:To preliminarily explore the mechanism of Yiaikang Capsules in the treatment of AIDS immune deficiency syndrome by UHPLC metabolomics technology.Methods:A total of 83 PIR patients were recruited in the study,including 25 patients without lung-spleen qi deficiency syndrome and 58 patients with lung-spleen qi deficiency syndrome.Intervention with Yiaikang Capsules for 24 weeks in patients with poor reconstruction of lung qi deficiency syndrome,Using UHPLC metabolomics technology to detect the different of each group,analyzing metabolomics data,screening differential metabolites,and useing enrichment and topological analysis to search for differential metabolic pathways.Results:CD_(4)^(+)T cell count,the expression rate of CD_(4)^(+)/CD_(8)^(+)were significantly increased(P<0.05).A total of 296 differential metabolites were finally identified in the lung-spleen qi deficiency syndrome group and the without lung-spleen qi deficiency syndrome group;248differential metabolites were finally identified after intervention.Enrichment analysis of metabolic pathways was conducted on differential metabolites,the common differential metabolic pathways between the lung-spleen qi deficiency group-the nonlung-spleen qi deficiency group,and before and after treatment with Yiaikang Capsules,were selenium amino acid metabolism,pantothenic acid and Co A biosynthesis,phosphoinositide metabolism,steroid hormone synthesis,nicotinic acid ester and nicotinamide metabolism,and purine metabolism.The metabolites hit by the common metabolic pathway are 1-phosphatidyl-D-methioinositol,PC[18:3(9Z,12Z,15Z)/20:1(11Z)],PE(14:0/P-18:0),quinolinic acid,deoxycorticosterone,and selenocysteine.Conclusion:Yiaikang Capsules can promote immune function reconstruction.1-phosphatidyl-D-methioinositol,PC[18:3(9Z,12Z,15Z)/20:1(11Z)],PE[14:0/P-18:0],quinolinic acid,deoxycorticosterone,selenocysteine,etc.are closely related to the therapeutic effect of Yiaikang Capsules on lung spleen qi deficiency syndrome.The metabolic pathway may be a potential entr

关 键 词：艾滋病 免疫重建不良 代谢组学 益艾康胶囊 

分 类 号：R259[医药卫生—中西医结合]