抑制Wnt/β-catenin信号通路对始初小头虫再生的影响  

Experiment study on the effect of inhibition of Wnt/β-catenin signaling on the regeneration of Capitella teleta

作　　者：李倩 张琳琳[1,2,3,4] LI Qian;ZHANG Linlin(Key Laboratory of Experimental Marine Biology,Institute of Oceanography,Chinese Academy of Sciences,Qingdao 266071,Shandong,China;Oceanographic Research Center,Chinese Academy of Sciences,Qingdao 266071,Shandong,China;Marine Biology and Biotechnology Functional Laboratory,Qingdao,266237,Shandong,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院海洋大科学研究中心,山东青岛266071 [3]海洋生物与生物技术实验室,山东青岛266237 [4]中国科学院大学,北京100049

出　　处：《上海海洋大学学报》2024年第3期552-561,共10页Journal of Shanghai Ocean University

基　　金：国家自然科学基金面上项目(41976088);深圳华大生命科学研究院开放基金(BGIRSZ20210013);中国科学院B类战略先导科技专项(XDB42000000);青岛海洋科学与技术试点国家实验室山东省专项经费(2022QNLM030004)。

摘　　要：Wnt/β-catenin信号通路在涡虫等多种生物的再生中发挥重要作用,本研究以环节动物始初小头虫(Capitella teleta)为研究对象,初步探究Wnt/β-catenin信号通路对小头虫再生的影响。将截断损伤后的样本持续浸泡于30μmol/L浓度外源性Wnt/β-catenin信号通路小分子化合物抑制剂XAV-939中,发现小头虫的再生受到了抑制,与对照组相比再生速度明显减慢,且不能形成正常的再生芽基组织,通过神经和肌肉标记发现Wnt信号通路被抑制后,小头虫再生过程中的神经和肌肉生长也受到了影响。后续通过EdU(5-Ethynyl-2’-deoxyuridine)标记发现抑制剂处理组样本的增殖细胞数量显著少于正常组样本,表明Wnt/β-catenin信号通路被抑制后会影响小头虫再生过程中的增殖细胞产生,从而调控小头虫的再生过程。在几种典型物种中Wnt通路调控再生的比较研究表明Wnt信号通路在刺胞动物和冠轮动物全身再生中的功能相对保守。Wnt/β-catenin signaling plays an important role in regeneration in multiple species such as planaria.The study aimed to investigate the effect of Wnt/β-catenin signaling on the regeneration in the annelid,Capitella teleta.Sustained incubation of samples after amputation in 30μmol/L XAV-939,an exogenous Wnt/β-catenin signaling small molecular inhibitor,revealed that the regeneration of C.teleta was inhibited,with significantly slower regeneration compared to that in the control group and failure to form a regeneration blastema.Neural and muscle growth during regeneration were also affected by inhibition of Wnt signaling revealed by neural and muscle labeling.Subsequently,EdU labeling indicated that the number of proliferating cells in the XAV 939-treated group was significantly less than that in the control group,suggesting that the suppression of the Wnt/β-catenin signaling may have affected the proliferating cells during the regeneration of C.teleta,which also further demonstrating that the Wnt/β-catenin signaling may be involved in the regeneration of C.teleta,and play an important role in the early stage of regeneration.Comparative studies on the regulation of regeneration by wnt signaling in several typical species have shown that the Wnt signaling is relatively conserved in the whole-body regeneration of Cnidaria and Lophotrochozoa.

关 键 词：WNT/Β-CATENIN信号通路 环节动物 小头虫 Wnt通路抑制剂 再生 增殖细胞 

分 类 号：Q418[生物学—生理学]