Aberration correction for deformable-mirror-based remote focusing enables high-accuracy whole-cell super-resolution imaging  

作　　者：WEI SHI YINGCHUAN HE JIANLIN WANG LULU ZHOU JIANWEI CHEN LIWEI ZHOU ZEYU XI ZHEN WANG KE FANG YIMING LI 

机构地区：[1]Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Photonics Research》2024年第4期821-832,共12页光子学研究（英文版）

基　　金：National Natural Science Foundation of China(62375116);Shenzhen Medical Research Fund(B2302038);Key Technology Research and Development Program of Shandong(2021CXGC010212);Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20220818100416036,KQTD20200820113012029);Startup Grant from Southern University of Science and Technology。

摘　　要：Single-molecule localization microscopy(SMLM)enables three-dimensional(3D)investigation of nanoscale structures in biological samples,offering unique insights into their organization.However,traditional 3D super-resolution microscopy using high numerical aperture(NA)objectives is limited by imaging depth of field(DOF),restricting their practical application to relatively thin biological samples.Here,we developed a unified solution for thick sample super-resolution imaging using a deformable mirror(DM)which served for fast remote focusing,optimized point spread function(PSF)engineering,and accurate aberration correction.By effectively correcting the system aberrations introduced during remote focusing and sample aberrations at different imaging depths,we achieved high-accuracy,large DOF imaging(~8μm)of the whole-cell organelles[i.e.,nuclear pore complex(NPC),microtubules,and mitochondria]with a nearly uniform resolution of approximately 35 nm across the entire cellular volume.

关 键 词：enable MIRROR RESOLUTION 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] R318[自动化与计算机技术—计算机科学与技术]