番茄尖果脐突变体的生理特性及其候选基因分析  

Mapping of the Sharp Blossom-end Gene and Screening of Candidate Gene in Tomato

作　　者：刘泽营 孙帅[1] 刘志强 崔霞 李仁 LIU Zeying;SUN Shuai;LIU Zhiqiang;CUI Xia;LI Ren(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2024年第5期982-992,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(32120103010,32272729);中国农业科学院青年创新专项(Y2022QC05)。

摘　　要：在栽培番茄Money Maker(MM)背景下获得的尖果脐(sharp blossom-end,shb)突变体果实的果脐突出,果实较小,呈心形,果实纵横比增加,说明SHB调控番茄果实大小和形状。徒手切片和石蜡切片观察发现,shb突变体果皮较MM果皮细胞层数显著减少,果皮变薄。在花柱发育早期,MM花柱基部的细胞小而紧密,但shb突变体花柱细胞持续膨大,花柱基部不断扩张,并在脱落后形成尖果脐。以MM和shb为亲本杂交构建了F2代分离群体,遗传分析表明尖果脐表型是由隐性单基因控制的质量性状。将尖果脐和平果脐极端表型单株进行DNA混池,并利用BSA-seq(Bulked Segregant Analysis Coupled with Whole-Genome Sequencing)的方法初步筛选到4个候选基因。The sharp blossom-end mutant was obtained from cultivar tomato Money Maker(MM),named as shb.Fruits of shb mutants had a pointed tip and a predominantly increased fruit shape index,shown heart-shaped with smaller size,suggesting that SHB regulates tomato fruit size and shape.The freehand section and paraffin section observations showed that the pericarp thickness and cell layers of shb mutants were significantly decreased compared with MM.During early style development,different from the small and dense cells at the base of the MM style,the shb mutant style base and the cells at this region continued to expand,eventually forming a pointed end after style dropping.An F2 population which was derived from a cross between MM(flat blossom end)and shb(sharp blossom end)was generated.Genetic analysis indicated the sharp blossom end was a qualitative trait controlled by a single recessive gene.The individual plants with extreme fruit phenotypes of the pointed end and the flat one were divided into two groups for DNA pooling,and four candidate genes were preliminarily screened by using Bulked Segregant Analysis Coupled with Whole-Genome Sequencing(BSA-Seq).

关 键 词：番茄 果实脐部 果实形状 细胞分裂 

分 类 号：S641.2[农业科学—蔬菜学]