苹果轮纹病拮抗真菌Pa2的分离与鉴定  

Isolation and Identification of Endophytic Fungus Pa2 Antagonistic Against Apple Ring Rot Caused by Botryosphaeria dothidea

作　　者：史冰柯 王卓妮 覃艮红 黄天祥 王丽[1] 涂洪涛[1,3] 袁洪波 侯珲 SHI Bingke;WANG Zhuoni;QIN Genhong;HUANG Tianxiang;WANG Li;TU Hongtao;YUAN Hongbo;HOU Hui(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;Western Agricultural Research Center,Chinese Academy of Agricultural Sciences,Changji,XinJiang 831100,China;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang,Henan 453004,China)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]中国农业科学院西部农业研究中心,新疆昌吉831100 [3]中国农业科学院中原研究中心,河南新乡453004

出　　处：《园艺学报》2024年第5期1113-1125,共13页Acta Horticulturae Sinica

基　　金：新疆维吾尔自治区青年自然科学基金项目(2022D01B226);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-RIP);中国农业科学院郑州果树研究所科研业务费专项(ZGS202205,1610192023311);河南省重点研发与推广专项(232102110052)。

摘　　要：为了分离和筛选对苹果轮纹病菌(Botryosphaeria dothidea)有抑制效果的拮抗真菌,通过组织分离法从苹果轮纹病抗性品种的枝条上分离出内生真菌。结果从40个分离菌株中得到5个拮抗真菌菌株,其中鉴定为砖红镰刀菌(Fusarium lateritium)的Pa2菌株对苹果轮纹病的抑制效果最好,抑制率为77.49%。菌株Pa2上清液不仅可以破坏B.dothidea菌丝细胞膜,还可以诱导果实中致病相关基因MdPR1的表达。在苹果接种B.dothidea后5 d,菌株Pa2对苹果轮纹病的控制效果为63.63%。In order to isolate and screen antagonistic fungi against apple ring rot caused by Botryosphaeria dothidea,the present study isolated endophytic fungi from the branches of apple ring rot resistant cultivar by tissue isolation methods.The results showed that five antagonistic fungal strains were obtained from 40 isolated strains,among which strain Pa2,identified as Fusarium lateritium,had the best inhibitory effect on B.dothidea with 77.49%inhibition rate.Strain Pa2 supernatant treatment can not only destroy the hyphae cell membrane,but also induce pathogenesis-related gene(MdPR1)expression in apple fruit.The result showed that the control effect of strain Pa2 on apple ring rot was 63.63%at 5 dpi.

关 键 词：苹果轮纹病 拮抗真菌 砖红镰刀菌 防效 

分 类 号：S661.1[农业科学—果树学]