绛红褐链霉菌CC2-6抑制大白菜根肿病的效果及机制分析  

Biocontrol Efficacy and Mechanisms of Streptomyces purpeofuscus CC2-6 Against Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae

作　　者：王青 胡燕[2] 陈姗 陆景伟[2] 郑阳[2] 陶伟林[2] 孙现超[1] 周娜[2] 陈国康[1] WANG Qing;HU Yan;CHEN Shan;LU Jingwei;ZHENG Yang;TAO Weilin;SUN Xianchao;ZHOU Na;CHEN Guokang(College of Plant Protection,Southwest University,Chongqing 400716,China;Institute of Vegetable and Flower Research,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China;Forestry Technology Extension Station of Jiashan County,Jiashan,Zhejiang 314199,China)

机构地区：[1]西南大学植物保护学院,重庆400716 [2]重庆市农业科学院蔬菜花卉研究所,重庆401329 [3]浙江省嘉善县林业技术推广站,浙江嘉善314199

出　　处：《园艺学报》2024年第5期1137-1150,共14页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-25-G-40);重庆市技术创新与应用发展专项(CSTB2022TIAD-LDX0020)。

摘　　要：为筛选可抑制芸薹根肿菌(Plasm odiophora brassicae)的生防菌株,以棉花枯萎病尖孢镰刀菌(Fusarium oxysporum)为指示菌,通过平板对峙法筛选出优良菌株CC2-6,其抑制率为50.03%;该菌株发酵液能抑制根肿菌休眠孢子萌发及根肿菌根毛侵染,提前7 d接菌CC2-6对大白菜根肿病的盆栽防效为53.20%。同时,该菌株CC2-6对包括烟草叶斑病菌(Pestalotiopsis nicotiae)等9种病原菌均有抑制效果,其抑菌率在47.20%~76.19%,表明菌株CC2-6具有广谱抑菌活性。形态学观察及16S rDNA基因序列分析,初步鉴定菌株CC2-6为绛红褐链霉菌(Streptomyces purpeofuscus)。菌株CC2-6可产生蛋白酶、纤维素酶、几丁质酶,其发酵液可诱导大白菜中的PPO、POD、PAL活性增强,SA、JA含量升高,降低ET含量。GC-TOF/LC-QTOF/QE非靶标代谢组学检测结果表明,菌株CC2-6发酵液中含有潮霉素B(Hygromycin B)、喜树碱(Camptothecin)、2–苯乙醇(2-Phenylethanol)、柠檬酸(Citric acid)等抑菌活性物质,表明CC2-6可能通过产生上述化合物抑制根肿菌侵染寄主。In order to screen out biocontrol strains that can effectively inhibit Plasmodiophora brassicae,Fusarium oxysporum was used as the indicator fungus,and the strain CC2-6 was screened by plate confrontation method.The inhibitory rate was 50.03%.The fermentation broth of strain CC2-6 could inhibit the germination of resting spores of P.brassicae and the infection of P.brassicae from root hairs,and and the control efficacy of Chinese cabbage clubroot was 53.20%in pot trials.In addition,CC2-6 had inhibitory effects on nine pathogens including Pestalotiopsis nicotiae,and its inhibition rate rangeed more than 47.20%–76.19%,indicating that CC2-6 had broad-spectrum bacteriostatic activity.The strain CC2-6 was preliminarily identified as Streptomyces purpeofuscus by morphological observation and 16S rDNA gene sequence analysis.The strain CC2-6 could produce protease,cellulase and chitinase,and its fermentation broth could induce the increase of PPO,POD and PAL activities,the increase of SA and JA contents and the decrease of ET content in Chinese cabbage.The results of GC-TOF/LC-QTOF/QE non-target metabolomics showed that the fermentation broth of strain CC2-6 contained antagnostic active substances such as hygromycin B,camptothecin,2-phenylethanol and citric acid,indicating that CC2-6 might inhibit the infection of P.brassicae by metabolizing these compounds.

关 键 词：根肿菌 生防放线菌 绛红褐链霉菌 诱导抗性 代谢产物 

分 类 号：S634.1[农业科学—蔬菜学]