治疗放烧复合伤的龙油溶致液晶研究  

Loong oil-lyotropic liquid crystals for the treatment of combined radiation and burn injury

作　　者：郭婉婷 贾学丽 刘岩[2] 胡亚丹 王可 张蕾 张勇 金义光 GUO Wan-ting;JIA Xue-li;LIU Yan;HU Ya-dan;WANG Ke;ZHANG Lei;ZHANG Yong;JIN Yi-guang(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Anhui Longjiyuan Biotechnology Co.,Ltd.,Suzhou 234000,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]军事科学院军事医学研究院辐射医学研究所,北京100850 [3]安徽龙纪元生物科技有限公司,安徽宿州234000

出　　处：《药学学报》2024年第5期1449-1457,共9页Acta Pharmaceutica Sinica

基　　金：北京市自然科学基金面上项目(7192148)。

摘　　要：放烧复合伤(combined radiation and burn injury,CRBI)是在受到放射性损伤的同时或相继发生烧伤的一种复合伤,临床缺乏有效治疗药物。龙油(Loong oil,LO)是由海螵蛸、红花、核桃油和菜籽油组成的中药油浸物,具有抗辐射和促进组织生长的作用。本研究使用单油酸甘油酯制备溶致液晶并包载LO,得到龙油溶致液晶(Loong oil-lyotropic liquid crystals,LOL),用于皮肤CRBI的治疗。LOL在X射线小角散射中的峰值比约■,偏光显微镜下呈现双折射现象,为六角相液晶结构。LOL中LO含量为4%,载药量高,生物黏附性好,适合皮肤给药。所有动物实验经军事科学院军事医学研究院辐射医学研究所伦理委员会批准且实验均按照相关指导原则和规定进行(批准号:IACUC-DWZX-2022-834)。与三乙醇胺乳膏相比,LOL皮肤渗透性显著增加,皮肤滞留性强,有利于深度CRBI的治疗。小鼠5 Gy ^(60)Co γ射线全身辐照后100℃烫伤6 s,成功构建深II度CRBI模型。LOL促进表皮细胞增殖及毛囊生成,加速伤口愈合并抑制瘢痕的发生,对CRBI有良好的治疗效果。本研究扩大了LO的应用范围,LOL是一种潜在的CRBI治疗药品。Combined radiation and burn injury(CRBI) is a severe syndrome,which is induced by the simultaneous or successive radiation and burn;but no appropriate clinical therapies are available.Loong oil(LO) is a traditional Chinese medicine oil composed of the oil extracts of cuttlebone,safflower,walnut oil,and rapeseed oil,which has been demonstrated to own anti-radiation and tissue healing functions.In this study,glyceryl monostearate(GMO) was used for the preparation of lyotropic liquid crystals that loaded LO to obtain Loong oillyotropic liquid crystals(LOL) for the treatment of skin CRBI.The hexagonal phase structure of LOL was proved by small X-ray scattering(SAXS) analysis with an approximate ■ ratio of peaks and under the polarizing microscope with the birefringence phenomenon.LOL had a high LO loading capacity(4%) and good bio-adhesive force,favoring skin administration.Animal experiments were approved by the Ethics Committee of the Beijing Institute of Radiation Medicine,Academy of Military Medical Sciences and the experiments were conducted in accordance with relevant guidelines and regulations(approval number:IACUC-DWZX-2022-834).Compared to triethanolamine creams,LOL had higher skin permeability with total skin penetration within 1 h,benefiting the treatment of deep CRBI.A deep II degree burn CRBI mouse model was established after wholebody irradiation with 5 Gy ~(60)Co γ-ray and the next 6-second 100 ℃ burn injury.LOL promoted LO skin penetration,improved epithermal cell proliferation and the formation of hair follicles,accelerated wound healing,and inhibited scarring,which benefitted the therapy of CRBI.In this study,LO application fields are expanded and LO is a promising medication for the treatment of CRBI.

关 键 词：放烧复合伤 龙油 溶致液晶 电离辐射 皮肤损伤 

分 类 号：R943[医药卫生—药剂学]