山东道地药材金银花不同发育阶段次生代谢产物含量动态变化研究及抗氧化活性比较  

Content of secondary metabolites and antioxidant activities of Lonicera japonica flowers at different developmental stages from Shandong province

作　　者：寇雁南 张旭[1,3] 孔富康 董红静 杨健 王维皓[1] 孔德志[2] 杨滨[1] KOU Yan-nan;ZHANG Xu;KONG Fu-kang;DONG Hong-jing;YANG Jian;WANG Wei-hao;KONG De-zhi;YANG Bin(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Integrated Traditional Chinese and Western Medicine,Hebei Medical University,Shijiazhuang 050017,China;College of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China;Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Ji'nan 250014,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]河北医科大学中西医结合研究所,河北石家庄050017 [3]河北医科大学药学院,河北石家庄050017 [4]齐鲁工业大学(山东省科学院)山东省分析测试中心山东省大型精密分析仪器应用技术重点实验室,山东济南250014 [5]中国中医科学院中药资源中心,北京100700

出　　处：《中国中药杂志》2024年第10期2654-2665,共12页China Journal of Chinese Materia Medica

基　　金：山东省重点研发计划项目(2021CXGC010508)。

摘　　要：该研究建立了金银花中环烯醚萜、酚酸、黄酮等11个活性成分的波长提取-高效液相多指标同步含量测定方法;采集山东2个金银花种植基地的四季花和北花一号从米蕾期(ML)到金花期(JH)6个不同发育阶段的金银花花蕾。运用建立的含量测定方法,测定样品中11个指标成分的含量,研究不同品种的金银花活性成分含量随发育阶段的变化规律;并以相关性分析的方法,研究环烯醚萜、酚酸和黄酮含量变化的相关性,挖掘3类成分在植物体内生物合成方面可能存在的关联;通过体外抗氧化活性实验,考察不同发育阶段样品的抗氧化活性,经相关性分析,挖掘抗氧化活性与化学成分的关系。研究结果显示:从米蕾期(ML)到金花期(JH),四季花中11个成分的含量总和呈“W”型变化,ML和二白期(EB)是3类成分含量较高的时期,ML最高;北花一号中11成分的含量总和自ML始,逐渐降低,ML是3类成分含量最高的时期。四季花中环烯醚萜含量高于北花一号,北花一号中酚酸含量高于四季花。四季花中黄酮和酚酸的含量变化存在明显的正相关(R~2=0.90,P<0.05);北花一号中,这2类成分含量变化不具有相关性。北花一号和四季花的抗氧化活性和酚酸含量存在明显的正相关:北花一号不同发育阶段酚酸含量与抗氧化活性的相关系数R~2=0.84 (P<0.05);四季花的相关系数R~2=0.73(P<0.05)。四季花和北花一号抗氧化能力最强的时期均为ML,其次EB。该研究揭示了金银花山东道地品种四季花和北花一号环烯醚萜、酚酸、黄酮的含量随发育阶段的变化规律,并证明金银花的抗氧化活性与不同发育阶段酚酸含量存在显著的相关性,为金银花最佳采收期的确定提供了依据。This study established an ultrasound-assisted extraction-high performance liquid chromatography method for simulta-neously determinining the content of 11 bioactive compounds including iridoids,phenolic acids,and flavonoids in Lonicera japonica flowers.The flowers at six stages from the rice bud stage(ML) to the golden flower stage(JH) of L.japonica varieties ′Sijuhua′ and ′Beihua No.1′ in two planting bases in Shandong province were collected.The established method was employed to determine the content of 11 target compounds,on the basis of which the dynamics of active components in L.japonica sampels during different development stages was investigated.The correlation analysis was carried out to reveal the correlations of the content of iridoids,phenolic acids,and flavonoids.Furthermore,the antioxidant activities of samples at different developmental stages were determined,and the relationship between antioxidant activity and chemical components was analyzed by the correlation analysis.The results showed that the total content of the 11 components in ′Sijihua′ changed in a "W" pattern from the ML to JH,being the highest at the ML and the second at the slight white stage(EB).The total content of 11 compounds in ′Beihua No.1′ was the highest at the ML and decreased gra-dually from the ML to JH.The samples of ′Sijihua′ had higher content of iridoids and lower content of phenolic acids than those of ′Beihua No.1′.The content of flavonoids and phenolic acids showed a positive correlation(R~2=0.90,P<0.05) in ′Sijihua′ but no obvious correlation in ′Beihua No.1′.The antioxidant activity and phenolic acid content showed positive correlations,with the determination coefficients(R~2) of 0.84(P<0.05) in ′Beihua No.1′ and 0.73(P<0.05) in ′Sijihua′.The antioxidant activity of both varieties was the strongest at the ML and the second at the EB.This study revealed that the content dynamics of iridoids,phenolic acids,and flavonoids in ′Sijihua′ and ′Beihua No.1′ cultivated in Sha

关 键 词：金银花 发育期 相关性分析 环烯醚萜 酚酸 黄酮 

分 类 号：R284.1[医药卫生—中药学]