Normal sperm head morphometric reference values in fertile Asian males  

作　　者：Ye-Lin Jia Ying-Bi Wu Lin Yu Yan Zheng Ting-Ting Yang Yan-Yun Wang Bin Zhou Lin Zhang Fu-Ping Li 

机构地区：[1]Laboratory of Andrology,West China Second University Hospital Human Sperm Bank,Chengdu 610000,China [2]Laboratory of Molecular Translational Medicine,Center for Translational Medicine,Chengdu 610061,China [3]Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University),Ministry of Education,Chengdu 610061,China [4]NHC Key Laboratory of Chronobiology,West China Second University Hospital,Sichuan University,Chengdu 610000,China

出　　处：《Asian Journal of Andrology》2024年第3期315-320,共6页亚洲男性学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81974226,No.32171264,and No.81974365);the Sichuan Science and Technology Program(No.2021YFS0026 and No.2022YFS0045).

摘　　要：Sperm head morphology is crucial for male factor infertility diagnosis and assessment of male reproductive potential.Severalcriteria are available to analyze sperm head morphology,but they are limited by poor methodology comparability and populationapplicability.This study aimed to explore comprehensive and new normal morphometric reference values for spermatozoa heads infertile Asian males.An automated sperm morphology analysis system captured 23152 stained spermatozoa from confirmed fertilemales.Of these samples,1856 sperm head images were annotated by three experienced laboratory technicians as“normal”.Weemployed 14 novel morphometric features to describe sperm head size(head length,head width,length/width ratio,and girth),shape(ellipse intersection over union,girth intersection over union,short-axis symmetry,and long-axis symmetry),area(head,acrosome,postacrosomal areas,and acrosome area ratio),and degrees of acrosome and nuclear uniformity.This straight-forwardmethod for the morphometric analysis of sperm by accurate visual measurements is clinically applicable.The measured parameterspresent valuable information to establish morphometric reference intervals for normal sperm heads in fertile Asian males.Thepresented detailed measurement data will be valuable for interlaboratory comparisons and technician training.In vitro fertilizationand andrology laboratory technicians can use these parameters to perform objective morphology evaluation when assessing malefertilization potential.

关 键 词：fertile Asian males head morphometric reference value normal spermatozoa 

分 类 号：R698.2[医药卫生—泌尿科学]