猪繁殖与呼吸综合征病毒RNA依赖的RNA聚合酶功能研究进展  

Progress in research on the function of porcine reproductive and respiratory syndrome virus RNA-dependent RNA polymerase

作　　者：段宏勇 唐超智[1] 王树茂 王安冉 姜一峰[2,3] 周艳君 高飞[2,3] 李丽薇 DUAN Hongyong;TANG Chaozhi;WANG Shumao;WANG Anran;JIANG Yifeng;ZHOU Yanjun;GAO Fei;LI Liwei(College of Life Science,Henan Normal University,Xinxiang 453007,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Scieces,Shanghai 200241,China;Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]中国农业科学院上海兽医研究所,上海200241 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2024年第5期674-679,共6页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2022YFD1800305,2022YFD1800804);上海市科技兴农项目(2022-02-08-00-12-F01162);中国农业科学院科技创新工程项目(CAAS-CSLPDCP-202301)。

摘　　要：猪繁殖与呼吸综合征(porcine reproductive and respiratory synd,rPoRmReS)是由猪繁殖与呼吸综合征病毒(PRRS virus,PRRSV)引起的一种重要猪病毒性传染病,其主要临床特征是母猪繁殖障碍和生长肥育猪呼吸系统疾病,给全球养猪业造成巨大损失。Nsp9是PRRSV的一种非结构蛋白,由于其RNA依赖的RNA聚合酶(RNA-dependent RNA polymerase,RdRp)功能,在PRRSV的复制和毒力中发挥着重要作用。Nsp9序列高度保守,并包含T细胞表位,对未来疫苗的开发具有重要意义。Nsp9在PRRSV感染过程中,特别是基因组RNA复制和转录中,成为病毒与宿主蛋白相互作用的中心。本文就Nsp9在病毒遗传变异、病毒毒力、与其他病毒蛋白及宿主蛋白相互作用影响病毒复制、宿主免疫调节、抗病毒药物及疫苗研发等方面的研究进展进行综述。本综述可为PRRSV的防控提供新的思路和策略。Porcine reproductive and respiratory syndrome(PRRS)is an important porcine infectious disease caused by the porcine reproductive and respiratory syndrome virus(PRRSV).Its main clinical features are reproductive disorders in sows and respiratory diseases in growing and finishing pigs,causing huge losses to the global pig industry.Nsp9 is a non-structural protein of PRRSV that plays an important role in the replication and virulence of PRRSV due to its RNA-dependent RNA polymerase(RdRp)function.The Nsp9 is important for the development of future vaccines,because its sequence is highly conserved and contains a T cell epitope.Nsp9 becomes the center of interaction between virus and host proteins during PRRSV infection,especially in genomic RNA replication and transcription.Herein,we summarize the progress of Nsp9 in research on the genetic variation,viral virulence,interactions with other viral proteins and host proteins affecting viral replication,host immune response regulation,the development of antiviral drugs and vaccines,which will provide novel ideas and strategies for the prevention and control of PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒(PRRSV) RNA依赖的RNA聚合酶(RdRp) 病毒复制 病毒毒力 疫苗研发 

分 类 号：S852.659.6[农业科学—基础兽医学]