苗药血人参提取物水凝胶的制备及促创面愈合体内外研究  

Preparation of a hydrogel of Indigofera stachyodes extract from Miao medicine and promotion of wound healing in vivo and ex vivo study

作　　者：宋选飞 杨露 谢欢 周斌 刘耀 徐剑 张永萍 SONG Xuanfei;YANG Lu;XIE Huan;ZHOU Bin;LIU Yao;XU Jian;ZHANG Yongping(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Ethnic Medicine Transdermal Drug Delivery Preparation Engineering Technology Research Center,Guiyang 550025,China;National Engineering Research Center of Miao’s Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州省民族药经皮给药制剂工程技术研究中心,贵州贵阳550025 [3]国家苗药工程技术研究中心,贵州贵阳550025

出　　处：《中草药》2024年第9期2933-2945,共13页Chinese Traditional and Herbal Drugs

基　　金：贵州省高等学校中药民族药(苗药)新剂型新制剂工程研究中心(黔教技[2022]022);国家苗药工程技术研究中心能力提升(黔科合中引地[2023]006);贵州省高层次创新型人才(黔科合平台人才-GCC[2023]037)。

摘　　要：目的制备一种载苗药血人参Indigofera stachyodes提取物的聚乙烯醇(PVA)/木质素(Lig)/壳聚糖(CS)水凝胶并探究其促创面愈合机制。方法在单因素考察的基础上,正交试验优选处方工艺并对其进行质量评价和体外生物评价,通过建立大鼠全层皮肤损伤模型,评价其促创愈合效果。结果携载提取物的PVA/Lig/CS水凝胶的最优处方和制备工艺为PVA用量7.0 g、CS用量4.0 g、Lig用量0.5 g、冻融循环次数4次,每次冻结6 h。该凝胶具有多孔网状结构、良好的溶胀性、保湿性及一定的机械强度,同时具有良好的生物相容性、抑菌性以及有效抑制巨噬细胞细胞迁移,且抑制作用可能与时间呈正比。该水凝胶的体内药效学研究显示,其促创面愈合作用可能与减少创面组织炎性细胞浸润,促进巨噬细胞向M2型极化、增加毛血细管生成和纤维组织形成有关。结论制备的载血人参提取物的PVA/Lig/CS水凝胶剂制备工艺简单、稳定可行、具有良好的缓释性能,且对全层皮肤损伤具较好的治疗效果。Objective To prepare of a polyvinyl alcohol(PVA)/lignin(Lig)/chitosan(CS)hydrogel containing extracts of Indigofera stachyodes(IS),a Miao medicine,and investigate its promoting wound healing mechanism.Methods On the basis of single factor investigation,orthogonal test was performed to prefer the prescription process and to evaluate its quality and in vitro biological evaluation,and its promoting wound healing effect was evaluated by establishing a rat whole skin injury model.Results The optimal prescription and preparation process of PVA/Lig/CS hydrogels carrying the extracts were 7.0 g of PVA,4.0 g of CS,0.5 g of Lig,and four freeze-thaw cycles of 6 h each.The gel has a porous mesh structure,good swelling property,moisture retention and some mechanical strength,as well as good biocompatibility,bacteriostatic properties and effective inhibition of macrophage cell migration,and the inhibition effect may be proportional to time.The in vivo pharmacodynamic study of this hydrogel showed that its promoting wound healing effect may be related to the reduction of inflammatory cell infiltration in the wound tissue,promotion of macrophage polarisation towards the M2 type,and increase of capillary formation and fibrous tissue formation.Conclusion The prepared PVA/Lig/CS hydrogel containing IS extract is simple,stable and feasible to prepare,has good slow-release properties,and has better therapeutic effect on all-over skin injury.

关 键 词：血人参提取物 PVA/Lig/CS水凝胶 创面愈合 体外生物评价 药效学研究 

分 类 号：R283.6[医药卫生—中药学]